Detailed information of TA system TA044172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3703572..3704251
• Replicon: chromosome
• Accession: NZ_AP027926
• Organism: Escherichia fergusonii strain 30038

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: QUE84_RS17785
• Protein ID: WP_002431667.1
• Coordinates: 3703949..3704251 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE84_RS17780
• Protein ID: WP_000806440.1
• Coordinates: 3703572..3703913 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE84_RS17770 (3699358)	3699358..3700647	-	1290	WP_000102764.1	APC family permease	-
QUE84_RS17775 (3700954)	3700954..3703458	+	2505	WP_061348936.1	copper-exporting P-type ATPase CopA	-
QUE84_RS17780 (3703572)	3703572..3703913	-	342	WP_000806440.1	HigA family addiction module antitoxin	Antitoxin
QUE84_RS17785 (3703949)	3703949..3704251	-	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE84_RS17790 (3704384)	3704384..3705178	+	795	WP_046082796.1	TraB/GumN family protein	-
QUE84_RS17795 (3705382)	3705382..3705861	+	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
QUE84_RS17800 (3705885)	3705885..3706685	+	801	WP_240846615.1	hypothetical protein	-
QUE84_RS17805 (3706814)	3706814..3707185	+	372	WP_223689340.1	hypothetical protein	-
QUE84_RS17810 (3707223)	3707223..3708875	-	1653	WP_223689339.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T44172 WP_002431667.1 NZ_AP027926:c3704251-3703949 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T44172 NZ_AP027926:c3704251-3703949 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT44172 WP_000806440.1 NZ_AP027926:c3703913-3703572 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT44172 NZ_AP027926:c3703913-3703572 [Escherichia fergusonii]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAGCCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACAACTG
AAATGGCGTTTCGTCTGGCTAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACACAAGAAGAATTGGGACGGATTGAAACAGTGGCTGACTATTTGGCTCGCCGTGAAGA
GCGTGCAAAGAAGGTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8