Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 1348194..1348795 Replicon chromosome
Accession NZ_AP027926
Organism Escherichia fergusonii strain 30038

Toxin (Protein)


Gene name doc Uniprot ID F0JYA5
Locus tag QUE84_RS06500 Protein ID WP_001216044.1
Coordinates 1348415..1348795 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag QUE84_RS06495 Protein ID WP_001190712.1
Coordinates 1348194..1348415 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE84_RS06485 (1345177) 1345177..1346565 - 1389 WP_001207923.1 nitrate/nitrite transporter NarU -
QUE84_RS06490 (1346828) 1346828..1347808 + 981 WP_000019434.1 IS5-like element ISKpn26 family transposase -
QUE84_RS06495 (1348194) 1348194..1348415 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
QUE84_RS06500 (1348415) 1348415..1348795 + 381 WP_001216044.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
QUE84_RS06505 (1348800) 1348800..1348979 + 180 WP_000113018.1 hypothetical protein -
QUE84_RS06510 (1349007) 1349007..1350050 + 1044 WP_000648835.1 DUF968 domain-containing protein -
QUE84_RS06515 (1350139) 1350139..1350591 + 453 WP_001312282.1 late promoter-activating protein (Gp10) -
QUE84_RS06520 (1350678) 1350678..1351871 + 1194 WP_000219619.1 hypothetical protein -
QUE84_RS06525 (1351871) 1351871..1353355 + 1485 WP_000124159.1 hypothetical protein -
QUE84_RS06530 (1353577) 1353577..1353696 + 120 WP_071594056.1 ash family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1346828..1347808 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13544.28 Da        Isoelectric Point: 5.6343

>T44159 WP_001216044.1 NZ_AP027926:1348415-1348795 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T44159 NZ_AP027926:1348415-1348795 [Escherichia fergusonii]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT44159 WP_001190712.1 NZ_AP027926:1348194-1348415 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT44159 NZ_AP027926:1348194-1348415 [Escherichia fergusonii]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y6HMN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References