Detailed information of TA system TA043817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1316315..1317020
• Replicon: chromosome
• Accession: NZ_AP027408
• Organism: Escherichia coli strain EC507 isolate EC507

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: QUE45_RS07525
• Protein ID: WP_000539521.1
• Coordinates: 1316634..1317020 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QUE45_RS07520
• Protein ID: WP_001280945.1
• Coordinates: 1316315..1316644 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE45_RS07505 (1311472)	1311472..1312383	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
QUE45_RS07510 (1312561)	1312561..1314909	+	2349	WP_001515111.1	EAL domain-containing protein	-
QUE45_RS07515 (1314917)	1314917..1316245	+	1329	WP_113368125.1	GGDEF domain-containing protein	-
QUE45_RS07520 (1316315)	1316315..1316644	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
QUE45_RS07525 (1316634)	1316634..1317020	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE45_RS07530 (1317246)	1317246..1318571	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
QUE45_RS07535 (1318784)	1318784..1319167	+	384	WP_000497137.1	biofilm formation regulator BssR	-
QUE45_RS07540 (1319278)	1319278..1320393	+	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
QUE45_RS07545 (1320390)	1320390..1321016	-	627	WP_124037031.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T43817 WP_000539521.1 NZ_AP027408:c1317020-1316634 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T43817 NZ_AP027408:c1317020-1316634 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT43817 WP_001280945.1 NZ_AP027408:c1316644-1316315 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT43817 NZ_AP027408:c1316644-1316315 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6