Detailed information of TA system TA043809

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56658..57084
• Replicon: plasmid pEC507_1
• Accession: NZ_AP027407
• Organism: Escherichia coli strain EC507 isolate EC507

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUE45_RS00960
• Protein ID: WP_096937776.1
• Coordinates: 56658..56783 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	56860..57084 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE45_RS00930 (51671)	51671..51946	-	276	WP_000234736.1	hypothetical protein	-
QUE45_RS00935 (52557)	52557..52940	-	384	WP_001137412.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QUE45_RS00940 (53410)	53410..53916	+	507	WP_096839359.1	transglycosylase SLT domain-containing protein	-
QUE45_RS00945 (54213)	54213..54710	-	498	Protein_50	DUF932 domain-containing protein	-
QUE45_RS00950 (54777)	54777..55047	+	271	Protein_51	hypothetical protein	-
QUE45_RS00960 (56658)	56658..56783	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
QUE45_RS00965 (56725)	56725..56874	-	150	Protein_54	plasmid maintenance protein Mok	-
- (56860)	56860..57084	-	225	NuclAT_0	-	Antitoxin
- (56860)	56860..57084	-	225	NuclAT_0	-	Antitoxin
- (56860)	56860..57084	-	225	NuclAT_0	-	Antitoxin
- (56860)	56860..57084	-	225	NuclAT_0	-	Antitoxin
QUE45_RS00970 (56896)	56896..57084	+	189	WP_032189914.1	hypothetical protein	-
QUE45_RS00975 (57102)	57102..57509	-	408	Protein_56	plasmid SOS inhibition protein A	-
QUE45_RS00980 (57506)	57506..57940	-	435	WP_096839430.1	conjugation system SOS inhibitor PsiB	-
QUE45_RS00985 (58009)	58009..59976	-	1968	WP_096839429.1	ParB/RepB/Spo0J family partition protein	-
QUE45_RS00990 (60037)	60037..60270	-	234	WP_000005990.1	DUF905 family protein	-
QUE45_RS00995 (60327)	60327..60866	-	540	WP_096839428.1	single-stranded DNA-binding protein	-
QUE45_RS01000 (61125)	61125..61585	+	461	Protein_61	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD	1..77354	77354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T43809 WP_096937776.1 NZ_AP027407:c56783-56658 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T43809 NZ_AP027407:c56783-56658 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT43809 NZ_AP027407:c57084-56860 [Escherichia coli]
TCACACGGATTGCCCGTGAACTGGCTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin