Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4059229..4060064 Replicon chromosome
Accession NZ_AP027246
Organism Escherichia coli strain JNE120393

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7W4PV54
Locus tag QUF33_RS20230 Protein ID WP_000854821.1
Coordinates 4059229..4059606 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7MK11
Locus tag QUF33_RS20235 Protein ID WP_001285610.1
Coordinates 4059696..4060064 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF33_RS20195 (4054310) 4054310..4054915 + 606 WP_001255030.1 type II secretion system minor pseudopilin GspJ -
QUF33_RS20200 (4054912) 4054912..4055889 + 978 WP_000633239.1 type II secretion system minor pseudopilin GspK -
QUF33_RS20205 (4055886) 4055886..4057064 + 1179 WP_000094989.1 type II secretion system protein GspL -
QUF33_RS20210 (4057066) 4057066..4057602 + 537 WP_000942794.1 GspM family type II secretion system protein YghD -
QUF33_RS20215 (4057883) 4057883..4058725 - 843 WP_001280493.1 DUF4942 domain-containing protein -
QUF33_RS20220 (4058810) 4058810..4059007 - 198 WP_085949090.1 DUF957 domain-containing protein -
QUF33_RS20225 (4059035) 4059035..4059232 - 198 Protein_3945 DUF5983 family protein -
QUF33_RS20230 (4059229) 4059229..4059606 - 378 WP_000854821.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
QUF33_RS20235 (4059696) 4059696..4060064 - 369 WP_001285610.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
QUF33_RS20240 (4060144) 4060144..4060365 - 222 WP_000692350.1 DUF987 domain-containing protein -
QUF33_RS20245 (4060452) 4060452..4060928 - 477 WP_001186727.1 RadC family protein -
QUF33_RS20250 (4060944) 4060944..4061429 - 486 WP_000849580.1 antirestriction protein -
QUF33_RS20255 (4061484) 4061484..4062302 - 819 WP_001234620.1 DUF932 domain-containing protein -
QUF33_RS20260 (4062402) 4062402..4062635 - 234 WP_001119729.1 DUF905 family protein -
QUF33_RS20265 (4062714) 4062714..4063169 - 456 WP_000581504.1 IrmA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - rfaE / gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM 3937892..4135072 197180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(19-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 13930.95 Da        Isoelectric Point: 8.5163

>T43578 WP_000854821.1 NZ_AP027246:c4059606-4059229 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T43578 NZ_AP027246:c4059606-4059229 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13606.39 Da        Isoelectric Point: 6.0618

>AT43578 WP_001285610.1 NZ_AP027246:c4060064-4059696 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT43578 NZ_AP027246:c4060064-4059696 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W4PV54


Antitoxin

Source ID Structure

References