Detailed information of TA system TA043570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2027337..2028174
• Replicon: chromosome
• Accession: NZ_AP027246
• Organism: Escherichia coli strain JNE120393

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUF33_RS10585
• Protein ID: WP_000227784.1
• Coordinates: 2027632..2028174 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QUF33_RS10580
• Protein ID: WP_001297137.1
• Coordinates: 2027337..2027648 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF33_RS10555 (2022357)	2022357..2023304	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
QUF33_RS10560 (2023326)	2023326..2025317	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUF33_RS10565 (2025307)	2025307..2025921	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUF33_RS10570 (2025921)	2025921..2026250	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUF33_RS10575 (2026262)	2026262..2027152	+	891	WP_000971336.1	heme o synthase	-
QUF33_RS10580 (2027337)	2027337..2027648	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QUF33_RS10585 (2027632)	2027632..2028174	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUF33_RS10590 (2028230)	2028230..2029165	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
QUF33_RS10595 (2029573)	2029573..2030937	+	1365	WP_001000978.1	MFS transporter	-
QUF33_RS10600 (2031065)	2031065..2031556	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUF33_RS10605 (2031724)	2031724..2032635	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43570 WP_000227784.1 NZ_AP027246:2027632-2028174 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43570 NZ_AP027246:2027632-2028174 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43570 WP_001297137.1 NZ_AP027246:2027337-2027648 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43570 NZ_AP027246:2027337-2027648 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3