Detailed information of TA system TA043532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 491819..492656
• Replicon: chromosome
• Accession: NZ_AP027217
• Organism: Escherichia coli strain JNE170426

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUE10_RS02245
• Protein ID: WP_000227784.1
• Coordinates: 491819..492361 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A3T7ZCX8
• Locus tag: QUE10_RS02250
• Protein ID: WP_001442339.1
• Coordinates: 492345..492656 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE10_RS02225 (487358)	487358..488269	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
QUE10_RS02230 (488437)	488437..488928	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE10_RS02235 (489056)	489056..490420	-	1365	WP_001000983.1	MFS transporter	-
QUE10_RS02240 (490828)	490828..491763	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
QUE10_RS02245 (491819)	491819..492361	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUE10_RS02250 (492345)	492345..492656	-	312	WP_001442339.1	DUF1778 domain-containing protein	Antitoxin
QUE10_RS02255 (492841)	492841..493731	-	891	WP_000971336.1	heme o synthase	-
QUE10_RS02260 (493743)	493743..494072	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE10_RS02265 (494072)	494072..494686	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUE10_RS02270 (494676)	494676..496667	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE10_RS02275 (496689)	496689..497636	-	948	WP_001239444.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43532 WP_000227784.1 NZ_AP027217:c492361-491819 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43532 NZ_AP027217:c492361-491819 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43532 WP_001442339.1 NZ_AP027217:c492656-492345 [Escherichia coli]
MPGKTATLPDVDKTLKNARVEIKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43532 NZ_AP027217:c492656-492345 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAAATCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A3T7ZCX8