Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1688372..1689026 Replicon chromosome
Accession NZ_AP027206
Organism Escherichia coli strain JNE082640

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag QUE04_RS08140 Protein ID WP_000244772.1
Coordinates 1688619..1689026 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE04_RS08135 Protein ID WP_000354046.1
Coordinates 1688372..1688638 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE04_RS08110 (1683660) 1683660..1684403 + 744 WP_000951965.1 SDR family oxidoreductase -
QUE04_RS08115 (1684460) 1684460..1685893 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
QUE04_RS08120 (1685938) 1685938..1686249 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QUE04_RS08125 (1686413) 1686413..1687072 + 660 WP_000250274.1 hemolysin III family protein -
QUE04_RS08130 (1687149) 1687149..1688129 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUE04_RS08135 (1688372) 1688372..1688638 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE04_RS08140 (1688619) 1688619..1689026 + 408 WP_000244772.1 protein YgfX Toxin
QUE04_RS08145 (1689066) 1689066..1689587 - 522 WP_001055874.1 flavodoxin FldB -
QUE04_RS08150 (1689699) 1689699..1690595 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE04_RS08155 (1690620) 1690620..1691330 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE04_RS08160 (1691336) 1691336..1693069 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T43487 WP_000244772.1 NZ_AP027206:1688619-1689026 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43487 NZ_AP027206:1688619-1689026 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43487 WP_000354046.1 NZ_AP027206:1688372-1688638 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43487 NZ_AP027206:1688372-1688638 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References