Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 489024..489642 Replicon chromosome
Accession NZ_AP027156
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUD66_RS02255 Protein ID WP_001291435.1
Coordinates 489024..489242 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUD66_RS02260 Protein ID WP_000344800.1
Coordinates 489268..489642 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS02220 (484313) 484313..484885 + 573 WP_000779831.1 YbaY family lipoprotein -
QUD66_RS02225 (484916) 484916..485227 - 312 WP_000409911.1 MGMT family protein -
QUD66_RS02235 (485606) 485606..485959 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QUD66_RS02240 (486001) 486001..487551 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUD66_RS02245 (487715) 487715..488185 - 471 WP_000136192.1 YlaC family protein -
QUD66_RS02250 (488301) 488301..488852 - 552 WP_000102568.1 maltose O-acetyltransferase -
QUD66_RS02255 (489024) 489024..489242 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUD66_RS02260 (489268) 489268..489642 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUD66_RS02265 (490188) 490188..493337 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QUD66_RS02270 (493360) 493360..494553 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43047 WP_001291435.1 NZ_AP027156:c489242-489024 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43047 NZ_AP027156:c489242-489024 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43047 WP_000344800.1 NZ_AP027156:c489642-489268 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43047 NZ_AP027156:c489642-489268 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References