Detailed information of TA system TA042965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 2164564..2165123
• Replicon: chromosome
• Accession: NZ_AP027050
• Organism: Vibrio nigripulchritudo strain TUMSAT-V.nig2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U4DSV3
• Locus tag: QMG91_RS28070
• Protein ID: WP_004404011.1
• Coordinates: 2164845..2165123 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U4DQE1
• Locus tag: QMG91_RS28065
• Protein ID: WP_004404015.1
• Coordinates: 2164564..2164848 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG91_RS28045 (TUMSATVNIG2_54980)	2160447..2161481	+	1035	WP_281831339.1	iron ABC transporter permease	-
QMG91_RS28050 (TUMSATVNIG2_54990)	2161478..2162455	+	978	WP_004404023.1	iron chelate uptake ABC transporter family permease subunit	-
QMG91_RS28055 (TUMSATVNIG2_55000)	2162460..2163257	+	798	WP_080927995.1	ATP-binding cassette domain-containing protein	-
QMG91_RS28060 (TUMSATVNIG2_55010)	2163508..2164341	+	834	WP_281831341.1	inositol monophosphatase	-
QMG91_RS28065 (TUMSATVNIG2_55020)	2164564..2164848	+	285	WP_004404015.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
QMG91_RS28070 (TUMSATVNIG2_55030)	2164845..2165123	+	279	WP_004404011.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
QMG91_RS28075 (TUMSATVNIG2_55040)	2165173..2165457	-	285	WP_022599002.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
QMG91_RS28080	2165538..2166161	-	624	WP_281832766.1	DUF998 domain-containing protein	-
QMG91_RS28085 (TUMSATVNIG2_55050)	2166336..2167091	-	756	WP_281831345.1	uroporphyrinogen-III C-methyltransferase	-
QMG91_RS28090 (TUMSATVNIG2_55060)	2167144..2167953	-	810	WP_281831347.1	formate/nitrite transporter family protein	-
QMG91_RS28095 (TUMSATVNIG2_55070)	2168065..2168397	-	333	WP_004403996.1	nitrite reductase small subunit NirD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (1-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-72)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10837.54 Da
• Isoelectric Point: 4.4400

>T42965 WP_004404011.1 NZ_AP027050:2164845-2165123 [Vibrio nigripulchritudo]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDSLIEAKVENLLEQPLMGVQRNGIRGRLLIIPEVSIIVPYWVEGEVIRIL
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T42965 NZ_AP027050:2164845-2165123 [Vibrio nigripulchritudo]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTTGAGTTTCTCTATGACTTCAATCCTGATGCAGC
AGAGAAAACCGACAGCCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAGCAACCTCTTATGGGCGTGCAAAGAAACGGAA
TCCGTGGACGTTTACTCATTATTCCTGAGGTATCAATAATCGTACCGTACTGGGTTGAAGGCGAAGTTATCCGCATTCTG
CGTGTGCTCCACCAAAAACAAAAATTCCCTACAGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11022.34 Da
• Isoelectric Point: 8.0517

>AT42965 WP_004404015.1 NZ_AP027050:2164564-2164848 [Vibrio nigripulchritudo]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKTLSHDAWLTEQVNLAFEKLDSGKSVFVEHESAKSR
MEERKARIRNRGKQ

Nucleotide

• Length: 285 bp

>AT42965 NZ_AP027050:2164564-2164848 [Vibrio nigripulchritudo]
ATGGACACTAGAATTCAGTTTCGTGTTGATGAGGAAACAAAACGCTTAGCTCAGCAAATGGCTGAAAGTCAGGGTCGTAC
ATTGAGTGATGCTTGCCGAGAACTTACTGAACAACTCGCTGAACAACAAAGAAAAACACTGTCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGCTTGACTCAGGAAAGTCGGTTTTCGTTGAACATGAATCGGCTAAATCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGCAACCGAGGTAAGCAATGA

Structures

Toxin

• AlphaFold DB: U4DSV3

Antitoxin

• AlphaFold DB: U4DQE1