Detailed information of TA system TA042849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3815845..3816442
• Replicon: chromosome
• Accession: NZ_AP026912
• Organism: Klebsiella pneumoniae strain Kp21_f22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: OO849_RS18855
• Protein ID: WP_004893639.1
• Coordinates: 3816125..3816442 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: OO849_RS18850
• Protein ID: WP_004142561.1
• Coordinates: 3815845..3816132 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO849_RS18820 (3812062)	3812062..3812310	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
OO849_RS18825 (3812328)	3812328..3812669	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
OO849_RS18830 (3812700)	3812700..3813815	-	1116	Protein_3690	MBL fold metallo-hydrolase	-
OO849_RS18835 (3813995)	3813995..3814566	+	572	Protein_3691	TetR/AcrR family transcriptional regulator	-
OO849_RS18840 (3814566)	3814566..3814934	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
OO849_RS18845 (3815054)	3815054..3815707	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
OO849_RS18850 (3815845)	3815845..3816132	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
OO849_RS18855 (3816125)	3816125..3816442	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OO849_RS18860 (3816627)	3816627..3817670	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
OO849_RS18865 (3818340)	3818340..3819206	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
OO849_RS18870 (3819315)	3819315..3820742	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T42849 WP_004893639.1 NZ_AP026912:c3816442-3816125 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T42849 NZ_AP026912:c3816442-3816125 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT42849 WP_004142561.1 NZ_AP026912:c3816132-3815845 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT42849 NZ_AP026912:c3816132-3815845 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1