Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1952174..1953005 Replicon chromosome
Accession NZ_AP026794
Organism Escherichia coli strain IPCEC48

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag OPA53_RS09255 Protein ID WP_000854815.1
Coordinates 1952174..1952548 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag OPA53_RS09260 Protein ID WP_001280918.1
Coordinates 1952637..1953005 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPA53_RS09220 (1947570) 1947570..1948736 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
OPA53_RS09225 (1948855) 1948855..1949328 + 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
OPA53_RS09230 (1949526) 1949526..1950584 + 1059 WP_001200889.1 FUSC family protein -
OPA53_RS09235 (1950756) 1950756..1951085 + 330 WP_000450409.1 DUF496 family protein -
OPA53_RS09240 (1951186) 1951186..1951542 - 357 WP_023142355.1 EutP/PduV family microcompartment system protein -
OPA53_RS09245 (1951857) 1951857..1951937 - 81 Protein_1817 hypothetical protein -
OPA53_RS09250 (1951983) 1951983..1952177 - 195 WP_000988600.1 DUF5983 family protein -
OPA53_RS09255 (1952174) 1952174..1952548 - 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
OPA53_RS09260 (1952637) 1952637..1953005 - 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
OPA53_RS09265 (1953021) 1953021..1953665 - 645 WP_000086752.1 hypothetical protein -
OPA53_RS09270 (1953684) 1953684..1953905 - 222 WP_000692345.1 DUF987 domain-containing protein -
OPA53_RS09275 (1953968) 1953968..1954444 - 477 WP_001186200.1 RadC family protein -
OPA53_RS09280 (1954460) 1954460..1954933 - 474 WP_001542276.1 antirestriction protein -
OPA53_RS09285 (1955027) 1955027..1955272 - 246 WP_001164966.1 hypothetical protein -
OPA53_RS09290 (1955272) 1955272..1956090 - 819 WP_001542275.1 DUF932 domain-containing protein -
OPA53_RS09295 (1956311) 1956311..1956721 - 411 WP_000846703.1 hypothetical protein -
OPA53_RS09300 (1956737) 1956737..1957087 - 351 Protein_1828 hypothetical protein -
OPA53_RS09305 (1957170) 1957170..1957916 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T42730 WP_000854815.1 NZ_AP026794:c1952548-1952174 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T42730 NZ_AP026794:c1952548-1952174 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT42730 WP_001280918.1 NZ_AP026794:c1953005-1952637 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT42730 NZ_AP026794:c1953005-1952637 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References