Detailed information of TA system TA042478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 46464..46989
• Replicon: plasmid pKAM622_3
• Accession: NZ_AP026395
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: P4I30_RS29135
• Protein ID: WP_004197633.1
• Coordinates: 46684..46989 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: P4I30_RS29130
• Protein ID: WP_004197642.1
• Coordinates: 46464..46682 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I30_RS29105 (KAM622c_57180)	42429..42608	-	180	WP_276086042.1	hypothetical protein	-
P4I30_RS29110 (KAM622c_57190)	42805..43083	+	279	WP_048295725.1	hypothetical protein	-
P4I30_RS29115 (KAM622c_57200)	43434..44138	+	705	WP_048295724.1	hypothetical protein	-
P4I30_RS29120 (KAM622c_57210)	44198..45130	-	933	WP_048295723.1	Abi family protein	-
P4I30_RS29125 (KAM622c_57220)	45314..45895	-	582	WP_011977803.1	hypothetical protein	-
P4I30_RS29130 (KAM622c_57230)	46464..46682	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
P4I30_RS29135 (KAM622c_57240)	46684..46989	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
P4I30_RS29140 (KAM622c_57250)	47158..47559	+	402	WP_072199347.1	hypothetical protein	-
P4I30_RS29145 (KAM622c_57260)	47586..47909	+	324	WP_004197641.1	hypothetical protein	-
P4I30_RS29150 (KAM622c_57270)	47906..48922	+	1017	WP_004197639.1	hypothetical protein	-
P4I30_RS29155 (KAM622c_57280)	49120..49914	+	795	WP_004197635.1	site-specific integrase	-
P4I30_RS29160	50370..50672	-	303	Protein_52	hypothetical protein	-
P4I30_RS29165 (KAM622c_57300)	50669..51295	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF	1..129931	129931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T42478 WP_004197633.1 NZ_AP026395:46684-46989 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T42478 NZ_AP026395:46684-46989 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT42478 WP_004197642.1 NZ_AP026395:46464-46682 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT42478 NZ_AP026395:46464-46682 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5