Detailed information of TA system TA042471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2672329..2673917
• Replicon: chromosome
• Accession: NZ_AP026392
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: P4I30_RS13520
• Protein ID: WP_044523012.1
• Coordinates: 2672329..2673651 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: P4I30_RS13525
• Protein ID: WP_023289667.1
• Coordinates: 2673651..2673917 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I30_RS13490 (2667375)	2667375..2669030	-	1656	WP_087776238.1	EAL domain-containing protein	-
P4I30_RS13495 (2669083)	2669083..2669331	-	249	Protein_2650	PAS domain-containing protein	-
P4I30_RS13500 (2669409)	2669409..2669576	+	168	Protein_2651	hypothetical protein	-
P4I30_RS13505 (2669868)	2669868..2670086	+	219	WP_002904808.1	ParD-like family protein	-
P4I30_RS13510 (2670067)	2670067..2670849	+	783	WP_218810086.1	type I methionyl aminopeptidase	-
P4I30_RS13515 (2671020)	2671020..2672312	+	1293	WP_261901442.1	glycoside hydrolase family 10 protein	-
P4I30_RS13520 (2672329)	2672329..2673651	-	1323	WP_044523012.1	type II toxin-antitoxin system HipA family toxin	Toxin
P4I30_RS13525 (2673651)	2673651..2673917	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
P4I30_RS13530 (2674181)	2674181..2674507	-	327	WP_217332803.1	hydroxyisourate hydrolase	-
P4I30_RS13535 (2674504)	2674504..2675004	-	501	WP_261901443.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
P4I30_RS13540 (2674982)	2674982..2676361	-	1380	WP_261901444.1	nucleobase:cation symporter-2 family protein	-
P4I30_RS13545 (2676707)	2676707..2677861	+	1155	WP_023289663.1	FAD-dependent urate hydroxylase HpxO	-
P4I30_RS13550 (2677842)	2677842..2678765	-	924	WP_241516047.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49183.49 Da
• Isoelectric Point: 8.6110

>T42471 WP_044523012.1 NZ_AP026392:c2673651-2672329 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T42471 NZ_AP026392:c2673651-2672329 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGACGAGTGGCTGCGCAGCCCGCGCGCCAGGCCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCACAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAACAGCCGTTCGATCTGCTGGCTGAGGTCGGCCGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCATCTTGCAGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAACTGACCGCGTTGCTGACCGCCTACCAGT
CGGATATTCCGCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATTGGCGAGAT
AAAACAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTAGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCACCACCCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGGCTGCCGCAAGAGGATCTGTGTCAGGCATTCGGCATCCCGTCGTCGATGAAATA
CGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAATTTCTCGCTCTTTCTACTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGAGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAACGCCACCCGAGGGCGGAAAACGGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTTCCCCGGGATCAGATGCTGGCGATCCTTGACGAGTTTGCCGACCGCGTGCCA
CAGGCCATCGAAAGCGCCCGCCGGACACTGCCGGCGGACTTTTCCGCCCACGTCTGGCGGGCGATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT42471 WP_023289667.1 NZ_AP026392:c2673917-2673651 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT42471 NZ_AP026392:c2673917-2673651 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCATTCCCCTCAGATATACAGTCCCGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCGGACAAAACCACCTTGA
CCACCCTATTCAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCCGAGACGGCGACGGCCCCC
GATAACACCACGGATCTGGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3