Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1868286..1868924 Replicon chromosome
Accession NZ_AP026116
Organism Escherichia coli strain CEC13002

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag OO850_RS09115 Protein ID WP_000813794.1
Coordinates 1868286..1868462 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag OO850_RS09120 Protein ID WP_001270286.1
Coordinates 1868508..1868924 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO850_RS09095 (1863907) 1863907..1865080 - 1174 Protein_1784 BenE family transporter YdcO -
OO850_RS09100 (1865172) 1865172..1865708 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
OO850_RS09105 (1865781) 1865781..1867742 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
OO850_RS09110 (1867834) 1867834..1868064 - 231 WP_000494244.1 YncJ family protein -
OO850_RS09115 (1868286) 1868286..1868462 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
OO850_RS09120 (1868508) 1868508..1868924 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
OO850_RS09125 (1869003) 1869003..1870409 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
OO850_RS09130 (1870654) 1870654..1871799 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
OO850_RS09135 (1871817) 1871817..1872830 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
OO850_RS09140 (1872831) 1872831..1873772 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T42391 WP_000813794.1 NZ_AP026116:1868286-1868462 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T42391 NZ_AP026116:1868286-1868462 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT42391 WP_001270286.1 NZ_AP026116:1868508-1868924 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT42391 NZ_AP026116:1868508-1868924 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References