Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3702039..3702693 Replicon chromosome
Accession NZ_AP026104
Organism Escherichia coli strain CEC96047

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag OO843_RS18635 Protein ID WP_000244781.1
Coordinates 3702039..3702446 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OO843_RS18640 Protein ID WP_000354046.1
Coordinates 3702427..3702693 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO843_RS18615 (3697996) 3697996..3699729 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
OO843_RS18620 (3699735) 3699735..3700445 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OO843_RS18625 (3700470) 3700470..3701366 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OO843_RS18630 (3701478) 3701478..3701999 + 522 WP_001055874.1 flavodoxin FldB -
OO843_RS18635 (3702039) 3702039..3702446 - 408 WP_000244781.1 protein YgfX Toxin
OO843_RS18640 (3702427) 3702427..3702693 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OO843_RS18645 (3702936) 3702936..3703916 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
OO843_RS18650 (3703993) 3703993..3704652 - 660 WP_000250274.1 hemolysin III family protein -
OO843_RS18655 (3704816) 3704816..3705127 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
OO843_RS18660 (3705172) 3705172..3706605 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T42180 WP_000244781.1 NZ_AP026104:c3702446-3702039 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T42180 NZ_AP026104:c3702446-3702039 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT42180 WP_000354046.1 NZ_AP026104:c3702693-3702427 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT42180 NZ_AP026104:c3702693-3702427 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References