Detailed information of TA system TA042125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54798..55348
• Replicon: chromosome
• Accession: NZ_AP026101
• Organism: Escherichia coli strain 93_161312

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: OO868_RS00250
• Protein ID: WP_001160975.1
• Coordinates: 55034..55348 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: OO868_RS00245
• Protein ID: WP_000125566.1
• Coordinates: 54798..55031 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO868_RS00220 (49872)	49872..50159	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
OO868_RS00225 (50217)	50217..51548	+	1332	WP_001183177.1	MFS transporter	-
OO868_RS00230 (51656)	51656..52186	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
OO868_RS00235 (52179)	52179..54041	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
OO868_RS00240 (54233)	54233..54712	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
OO868_RS00245 (54798)	54798..55031	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
OO868_RS00250 (55034)	55034..55348	+	315	WP_001160975.1	CcdB family protein	Toxin
OO868_RS00255 (55345)	55345..56193	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
OO868_RS00260 (56200)	56200..56577	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
OO868_RS00265 (56580)	56580..57401	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
OO868_RS00270 (57398)	57398..58387	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
OO868_RS00275 (58387)	58387..59673	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T42125 WP_001160975.1 NZ_AP026101:55034-55348 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T42125 NZ_AP026101:55034-55348 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT42125 WP_000125566.1 NZ_AP026101:54798-55031 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT42125 NZ_AP026101:54798-55031 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9