Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3917221..3917948 Replicon chromosome
Accession NZ_AP026085
Organism Escherichia coli strain H52_982342

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag OO840_RS19690 Protein ID WP_000550189.1
Coordinates 3917634..3917948 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag OO840_RS19685 Protein ID WP_000560249.1
Coordinates 3917221..3917637 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO840_RS19675 (3912281) 3912281..3914632 + 2352 WP_000695517.1 alpha-glucosidase -
OO840_RS19680 (3915158) 3915158..3917176 + 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
OO840_RS19685 (3917221) 3917221..3917637 - 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
OO840_RS19690 (3917634) 3917634..3917948 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
OO840_RS19695 (3918232) 3918232..3919368 - 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
OO840_RS19700 (3919453) 3919453..3919956 + 504 WP_001295542.1 M48 family metallopeptidase -
OO840_RS19705 (3920033) 3920033..3920725 + 693 WP_000942544.1 vancomycin high temperature exclusion protein -
OO840_RS19710 (3920804) 3920804..3921790 + 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T41893 WP_000550189.1 NZ_AP026085:c3917948-3917634 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T41893 NZ_AP026085:c3917948-3917634 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT41893 WP_000560249.1 NZ_AP026085:c3917637-3917221 [Escherichia coli]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT41893 NZ_AP026085:c3917637-3917221 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References