Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1880160..1880957 Replicon chromosome
Accession NZ_AP025851
Organism Enterobacter hormaechei strain FUJ80068

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag NGJ26_RS08960 Protein ID WP_032647926.1
Coordinates 1880436..1880957 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag NGJ26_RS08955 Protein ID WP_015570876.1
Coordinates 1880160..1880429 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGJ26_RS08920 (FJMB80068_17540) 1875700..1876059 + 360 WP_032647921.1 helix-turn-helix domain-containing protein -
NGJ26_RS08925 1876138..1876233 - 96 Protein_1737 transcriptional regulator -
NGJ26_RS08930 (FJMB80068_17560) 1876386..1877627 + 1242 WP_032647923.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
NGJ26_RS08935 (FJMB80068_17570) 1877660..1877887 - 228 WP_006809324.1 YccJ family protein -
NGJ26_RS08940 (FJMB80068_17580) 1877906..1878502 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
NGJ26_RS08945 (FJMB80068_17590) 1878894..1879064 + 171 WP_001273664.1 general stress protein -
NGJ26_RS08950 (FJMB80068_17600) 1879181..1880086 + 906 WP_017693666.1 DMT family transporter -
NGJ26_RS08955 (FJMB80068_17610) 1880160..1880429 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
NGJ26_RS08960 (FJMB80068_17620) 1880436..1880957 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
NGJ26_RS08965 (FJMB80068_17630) 1881016..1882338 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
NGJ26_RS08970 (FJMB80068_17640) 1882360..1882851 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
NGJ26_RS08975 (FJMB80068_17650) 1882864..1883454 - 591 WP_032647932.1 malonic semialdehyde reductase -
NGJ26_RS08980 (FJMB80068_17660) 1883464..1884264 - 801 WP_251268090.1 pyrimidine utilization protein D -
NGJ26_RS08985 (FJMB80068_17670) 1884272..1884658 - 387 WP_015570870.1 pyrimidine utilization protein C -
NGJ26_RS08990 (FJMB80068_17680) 1884670..1885359 - 690 WP_017384771.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T41371 WP_032647926.1 NZ_AP025851:1880436-1880957 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T41371 NZ_AP025851:1880436-1880957 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT41371 WP_015570876.1 NZ_AP025851:1880160-1880429 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT41371 NZ_AP025851:1880160-1880429 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References