Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1880161..1880958 Replicon chromosome
Accession NZ_AP025847
Organism Enterobacter hormaechei strain FUJ80063

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag NGK57_RS08960 Protein ID WP_032647926.1
Coordinates 1880437..1880958 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag NGK57_RS08955 Protein ID WP_015570876.1
Coordinates 1880161..1880430 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK57_RS08920 (FJMB80063_17550) 1875701..1876060 + 360 WP_032647921.1 helix-turn-helix domain-containing protein -
NGK57_RS08925 1876139..1876234 - 96 Protein_1737 transcriptional regulator -
NGK57_RS08930 (FJMB80063_17570) 1876387..1877628 + 1242 WP_032647923.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
NGK57_RS08935 (FJMB80063_17580) 1877661..1877888 - 228 WP_006809324.1 YccJ family protein -
NGK57_RS08940 (FJMB80063_17590) 1877907..1878503 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
NGK57_RS08945 (FJMB80063_17600) 1878895..1879065 + 171 WP_001273664.1 general stress protein -
NGK57_RS08950 (FJMB80063_17610) 1879182..1880087 + 906 WP_017693666.1 DMT family transporter -
NGK57_RS08955 (FJMB80063_17620) 1880161..1880430 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
NGK57_RS08960 (FJMB80063_17630) 1880437..1880958 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
NGK57_RS08965 (FJMB80063_17640) 1881017..1882339 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
NGK57_RS08970 (FJMB80063_17650) 1882361..1882852 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
NGK57_RS08975 (FJMB80063_17660) 1882865..1883455 - 591 WP_032647932.1 malonic semialdehyde reductase -
NGK57_RS08980 (FJMB80063_17670) 1883465..1884265 - 801 WP_251268090.1 pyrimidine utilization protein D -
NGK57_RS08985 (FJMB80063_17680) 1884273..1884659 - 387 WP_015570870.1 pyrimidine utilization protein C -
NGK57_RS08990 (FJMB80063_17690) 1884671..1885360 - 690 WP_017384771.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T41352 WP_032647926.1 NZ_AP025847:1880437-1880958 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T41352 NZ_AP025847:1880437-1880958 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT41352 WP_015570876.1 NZ_AP025847:1880161-1880430 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT41352 NZ_AP025847:1880161-1880430 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References