Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 68180..68434 Replicon plasmid pla2
Accession NZ_AP025756
Organism Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag QUE34_RS28295 Protein ID WP_001312851.1
Coordinates 68285..68434 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 68180..68241 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE34_RS28270 (65570) 65570..66316 + 747 WP_096912997.1 conjugal transfer pilus acetylase TraX -
QUE34_RS28275 (66383) 66383..66784 + 402 Protein_84 fertility inhibition protein FinO -
QUE34_RS28280 (66919) 66919..67070 + 152 Protein_85 hypothetical protein -
QUE34_RS28285 (67306) 67306..67392 + 87 Protein_86 endonuclease -
QUE34_RS28290 (67573) 67573..67884 - 312 WP_000765906.1 hypothetical protein -
- (68180) 68180..68241 - 62 NuclAT_0 - Antitoxin
- (68180) 68180..68241 - 62 NuclAT_0 - Antitoxin
- (68180) 68180..68241 - 62 NuclAT_0 - Antitoxin
- (68180) 68180..68241 - 62 NuclAT_0 - Antitoxin
QUE34_RS28295 (68285) 68285..68434 + 150 WP_001312851.1 Hok/Gef family protein Toxin
QUE34_RS28300 (68718) 68718..68975 + 258 WP_000083834.1 replication regulatory protein RepA -
QUE34_RS28305 (69078) 69078..69263 + 186 WP_039520370.1 plasmid copy control protein CopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..69275 69275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T40868 WP_001312851.1 NZ_AP025756:68285-68434 [Escherichia sp. KS167_9B]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T40868 NZ_AP025756:68285-68434 [Escherichia sp. KS167_9B]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 62 bp

>AT40868 NZ_AP025756:c68241-68180 [Escherichia sp. KS167_9B]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References