Detailed information of TA system TA040864

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 125080..125506
• Replicon: plasmid pla1
• Accession: NZ_AP025755
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUE34_RS27360
• Protein ID: WP_001372321.1
• Coordinates: 125080..125205 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	125282..125506 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS27330 (120633)	120633..121280	+	648	WP_000614283.1	transglycosylase SLT domain-containing protein	-
QUE34_RS27335 (121577)	121577..122398	-	822	Protein_147	DUF932 domain-containing protein	-
QUE34_RS27340 (122517)	122517..122804	-	288	WP_000107535.1	hypothetical protein	-
QUE34_RS27345 (123055)	123055..124059	+	1005	WP_024166184.1	IS110 family transposase	-
QUE34_RS27350 (124153)	124153..124413	-	261	WP_000547943.1	hypothetical protein	-
QUE34_RS27355 (124483)	124483..124655	+	173	Protein_151	hypothetical protein	-
QUE34_RS27360 (125080)	125080..125205	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
QUE34_RS27365 (125147)	125147..125296	-	150	Protein_153	plasmid maintenance protein Mok	-
- (125282)	125282..125506	-	225	NuclAT_0	-	Antitoxin
- (125282)	125282..125506	-	225	NuclAT_0	-	Antitoxin
- (125282)	125282..125506	-	225	NuclAT_0	-	Antitoxin
- (125282)	125282..125506	-	225	NuclAT_0	-	Antitoxin
QUE34_RS27370 (125318)	125318..125506	+	189	WP_001355331.1	hypothetical protein	-
QUE34_RS27375 (125451)	125451..126237	-	787	Protein_155	plasmid SOS inhibition protein A	-
QUE34_RS27380 (126234)	126234..126668	-	435	WP_000845944.1	conjugation system SOS inhibitor PsiB	-
QUE34_RS27385 (126737)	126737..128701	-	1965	WP_000117199.1	ParB/RepB/Spo0J family partition protein	-
QUE34_RS27390 (128765)	128765..128998	-	234	WP_000005990.1	DUF905 family protein	-
QUE34_RS27395 (129056)	129056..129595	-	540	WP_286338982.1	single-stranded DNA-binding protein	-
QUE34_RS27400 (129979)	129979..130236	-	258	WP_023147917.1	hypothetical protein	-
QUE34_RS27405 (130149)	130149..130362	-	214	Protein_161	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..215429	215429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T40864 WP_001372321.1 NZ_AP025755:c125205-125080 [Escherichia sp. KS167_9B]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T40864 NZ_AP025755:c125205-125080 [Escherichia sp. KS167_9B]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT40864 NZ_AP025755:c125506-125282 [Escherichia sp. KS167_9B]
TCACACGGATTGCCCGTGAACTGGCTGAACGACAAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGTAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin