Detailed information of TA system TA040852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3789238..3789965
• Replicon: chromosome
• Accession: NZ_AP025754
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2K3TXF2
• Locus tag: QUE34_RS18980
• Protein ID: WP_032303813.1
• Coordinates: 3789651..3789965 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE34_RS18975
• Protein ID: WP_000560266.1
• Coordinates: 3789238..3789654 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS18965 (3784599)	3784599..3786950	+	2352	WP_032303811.1	alpha-glucosidase	-
QUE34_RS18970 (3787175)	3787175..3789193	+	2019	WP_050009868.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
QUE34_RS18975 (3789238)	3789238..3789654	-	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
QUE34_RS18980 (3789651)	3789651..3789965	-	315	WP_032303813.1	type II toxin-antitoxin system toxin HigB	Toxin
QUE34_RS18985 (3790221)	3790221..3791357	-	1137	WP_000018675.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
QUE34_RS18990 (3791442)	3791442..3791945	+	504	WP_001295542.1	M48 family metallopeptidase	-
QUE34_RS18995 (3792023)	3792023..3792715	+	693	WP_032303814.1	vancomycin high temperature exclusion protein	-
QUE34_RS19000 (3792794)	3792794..3793780	+	987	WP_103253354.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12119.06 Da
• Isoelectric Point: 9.9056

>T40852 WP_032303813.1 NZ_AP025754:c3789965-3789651 [Escherichia sp. KS167_9B]
MHLITQKELKDAAEKYPQHKTELGALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T40852 NZ_AP025754:c3789965-3789651 [Escherichia sp. KS167_9B]
ATGCACCTGATAACTCAAAAAGAATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGGGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT40852 WP_000560266.1 NZ_AP025754:c3789654-3789238 [Escherichia sp. KS167_9B]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT40852 NZ_AP025754:c3789654-3789238 [Escherichia sp. KS167_9B]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCTGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A2K3TXF2