Detailed information of TA system TA040734

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 125738..126164
• Replicon: plasmid pla1
• Accession: NZ_AP025742
• Organism: Escherichia sp. 10290

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUE74_RS28070
• Protein ID: WP_001372321.1
• Coordinates: 125738..125863 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	125940..126164 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE74_RS28040 (121291)	121291..121938	+	648	WP_000614283.1	transglycosylase SLT domain-containing protein	-
QUE74_RS28045 (122235)	122235..123056	-	822	Protein_148	DUF932 domain-containing protein	-
QUE74_RS28050 (123175)	123175..123462	-	288	WP_000107535.1	hypothetical protein	-
QUE74_RS28055 (123713)	123713..124717	+	1005	WP_024166184.1	IS110 family transposase	-
QUE74_RS28060 (124811)	124811..125071	-	261	WP_000547943.1	hypothetical protein	-
QUE74_RS28065 (125141)	125141..125313	+	173	Protein_152	hypothetical protein	-
QUE74_RS28070 (125738)	125738..125863	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
QUE74_RS28075 (125805)	125805..125954	-	150	Protein_154	plasmid maintenance protein Mok	-
- (125940)	125940..126164	-	225	NuclAT_0	-	Antitoxin
- (125940)	125940..126164	-	225	NuclAT_0	-	Antitoxin
- (125940)	125940..126164	-	225	NuclAT_0	-	Antitoxin
- (125940)	125940..126164	-	225	NuclAT_0	-	Antitoxin
QUE74_RS28080 (125976)	125976..126164	+	189	WP_001355331.1	hypothetical protein	-
QUE74_RS28085 (126109)	126109..126895	-	787	Protein_156	plasmid SOS inhibition protein A	-
QUE74_RS28090 (126892)	126892..127326	-	435	WP_000845944.1	conjugation system SOS inhibitor PsiB	-
QUE74_RS28095 (127395)	127395..129359	-	1965	WP_000117199.1	ParB/RepB/Spo0J family partition protein	-
QUE74_RS28100 (129423)	129423..129656	-	234	WP_000005990.1	DUF905 family protein	-
QUE74_RS28105 (129714)	129714..130253	-	540	WP_000290857.1	single-stranded DNA-binding protein	-
QUE74_RS28110 (130637)	130637..130894	-	258	WP_023147917.1	hypothetical protein	-
QUE74_RS28115 (130807)	130807..131020	-	214	Protein_162	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..224339	224339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T40734 WP_001372321.1 NZ_AP025742:c125863-125738 [Escherichia sp. 10290]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T40734 NZ_AP025742:c125863-125738 [Escherichia sp. 10290]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT40734 NZ_AP025742:c126164-125940 [Escherichia sp. 10290]
TCACACGGATTGCCCGTGAACTGGCTGAACGACAAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGTAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin