Detailed information of TA system TA040196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 1605773..1606433
• Replicon: chromosome
• Accession: NZ_AP025256
• Organism: Paraburkholderia terrae strain KU-15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PTKU15_RS07365
• Protein ID: WP_079482156.1
• Coordinates: 1606005..1606433 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Z7B428
• Locus tag: PTKU15_RS07360
• Protein ID: WP_007588356.1
• Coordinates: 1605773..1606018 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PTKU15_RS07335 (PTKU15_14400)	1601364..1601876	-	513	WP_131241777.1	Spy/CpxP family protein refolding chaperone	-
PTKU15_RS07340 (PTKU15_14410)	1602029..1602913	+	885	WP_042314786.1	LysR family transcriptional regulator	-
PTKU15_RS07345 (PTKU15_14420)	1603051..1603515	+	465	WP_180722273.1	Lrp/AsnC family transcriptional regulator	-
PTKU15_RS07350 (PTKU15_14430)	1603723..1604820	+	1098	WP_042314784.1	saccharopine dehydrogenase C-terminal domain-containing protein	-
PTKU15_RS07355 (PTKU15_14440)	1604983..1605603	-	621	WP_248555850.1	ATP-binding protein	-
PTKU15_RS07360 (PTKU15_14450)	1605773..1606018	+	246	WP_007588356.1	DNA-binding protein	Antitoxin
PTKU15_RS07365 (PTKU15_14460)	1606005..1606433	+	429	WP_079482156.1	type II toxin-antitoxin system VapC family toxin	Toxin
PTKU15_RS07370 (PTKU15_14470)	1606524..1606997	+	474	WP_229513248.1	DUF2846 domain-containing protein	-
PTKU15_RS07375 (PTKU15_14480)	1607202..1608179	+	978	WP_042314781.1	magnesium/cobalt transporter CorA	-
PTKU15_RS07380 (PTKU15_14490)	1608222..1608860	-	639	WP_248555851.1	LysE family translocator	-
PTKU15_RS07385 (PTKU15_14500)	1609413..1610369	+	957	WP_042314779.1	sugar ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15778.96 Da
• Isoelectric Point: 6.8808

>T40196 WP_079482156.1 NZ_AP025256:1606005-1606433 [Paraburkholderia terrae]
VYLIDTNVISEMRKSSRANPGVQAFFETAKEGGHRLYLSVVTVGELRRGVDLIRHRGDKFQALRLEGWLDTIMNSFSSQI
LNVGTDVAQTWGHLRVPDPEHSLDKLIAATALIYNLTVVTRNVADFDGTGARVLNPFQLLDA

Nucleotide

• Length: 429 bp

>T40196 NZ_AP025256:1606005-1606433 [Paraburkholderia terrae]
GTGTATTTGATTGATACCAATGTAATCAGCGAAATGCGCAAGAGCAGCCGCGCCAATCCAGGCGTGCAGGCTTTTTTCGA
AACGGCGAAGGAAGGCGGGCATCGCCTGTATCTGTCGGTGGTGACGGTCGGCGAGCTACGGCGCGGCGTCGACCTGATCC
GGCATCGGGGCGACAAGTTCCAGGCGTTGCGCCTGGAAGGCTGGCTCGACACGATCATGAATTCGTTCAGCAGCCAGATC
CTGAATGTCGGCACCGACGTCGCGCAGACGTGGGGCCATCTGCGCGTGCCGGATCCCGAGCACTCGCTGGACAAGCTGAT
CGCGGCGACGGCCCTGATCTACAACCTGACTGTCGTGACGCGCAACGTGGCAGACTTCGATGGCACGGGCGCACGGGTGC
TGAATCCTTTCCAGCTTCTTGACGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8938.94 Da
• Isoelectric Point: 4.2208

>AT40196 WP_007588356.1 NZ_AP025256:1605773-1606018 [Paraburkholderia terrae]
MANLLVRNLDDALVQNLREQAAAHGRSVEAEHREILAQALFQPKKLSFAELLMSMPDVGDDADFERRNDVTDDSGGPGVF
D

Nucleotide

• Length: 246 bp

>AT40196 NZ_AP025256:1605773-1606018 [Paraburkholderia terrae]
ATGGCAAATCTTTTGGTCCGAAATCTGGACGATGCGTTGGTGCAGAACCTGCGTGAGCAGGCCGCGGCTCACGGGCGCAG
CGTCGAAGCAGAGCATCGGGAAATCCTGGCGCAAGCGCTCTTTCAGCCCAAGAAGCTCAGCTTCGCGGAATTGCTGATGA
GCATGCCCGACGTCGGCGACGACGCCGACTTCGAGCGCCGCAATGACGTAACTGACGATAGCGGGGGACCGGGTGTATTT
GATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z7B428