Detailed information of TA system TA040172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 62627..63218
• Replicon: chromosome
• Accession: NZ_AP025245
• Organism: Acidianus sp. HS-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HS5_RS00415
• Protein ID: WP_236752116.1
• Coordinates: 62850..63218 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HS5_RS00410
• Protein ID: WP_236752115.1
• Coordinates: 62627..62866 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS5_RS00370 (HS5_00690)	58010..58393	+	384	WP_236752107.1	hypothetical protein	-
HS5_RS00375 (HS5_00700)	58375..59829	-	1455	WP_236752108.1	MFS transporter	-
HS5_RS00380 (HS5_00710)	59943..60506	+	564	WP_236752109.1	isochorismatase family cysteine hydrolase	-
HS5_RS00385 (HS5_00720)	60498..61025	-	528	WP_236752110.1	hypothetical protein	-
HS5_RS00390 (HS5_00730)	60992..61237	-	246	WP_236752111.1	hypothetical protein	-
HS5_RS00395 (HS5_00740)	61253..61936	-	684	WP_236752112.1	class II glutamine amidotransferase	-
HS5_RS00400 (HS5_00750)	61953..62159	-	207	WP_236752113.1	CDGSH iron-sulfur domain-containing protein	-
HS5_RS00405 (HS5_00760)	62224..62535	+	312	WP_236752114.1	helix-turn-helix domain-containing protein	-
HS5_RS00410 (HS5_00770)	62627..62866	+	240	WP_236752115.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HS5_RS00415 (HS5_00780)	62850..63218	+	369	WP_236752116.1	PIN domain-containing protein	Toxin
HS5_RS00420 (HS5_00790)	63885..64313	+	429	WP_236752117.1	HEPN domain-containing protein	-
HS5_RS00425 (HS5_00800)	64276..64725	+	450	WP_236752118.1	nucleotidyltransferase domain-containing protein	-
HS5_RS00430 (HS5_00810)	64718..65122	+	405	WP_236752119.1	HEPN domain-containing protein	-
HS5_RS00435 (HS5_00820)	65092..65475	+	384	WP_236752120.1	nucleotidyltransferase domain-containing protein	-
HS5_RS00440 (HS5_00830)	65452..66339	-	888	WP_236752121.1	lysine exporter LysO family protein	-
HS5_RS00445 (HS5_00840)	66375..67265	-	891	WP_236752122.1	DUF973 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Antitoxin

• SpoVT-AbrB domain (4-45)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14104.39 Da
• Isoelectric Point: 4.8006

>T40172 WP_236752116.1 NZ_AP025245:62850-63218 [Acidianus sp. HS-5]
MEAVVDTNFIIAVVFKDHVDHEEAIKEWEKLDKAYLPLISVTEIAYFLIKHNIDLNVLSEILSDPKIEVVQNDVEDVYYA
LTKKSEIKGYDDFNDFLILSTAITQKLPLLTFDKKLKKKMGK

Nucleotide

• Length: 369 bp

>T40172 NZ_AP025245:62850-63218 [Acidianus sp. HS-5]
TTGGAAGCAGTAGTTGATACAAATTTCATAATTGCGGTAGTCTTTAAAGATCACGTTGATCATGAAGAGGCTATAAAAGA
ATGGGAAAAATTAGATAAGGCTTACTTACCCCTTATTTCAGTCACTGAAATAGCTTATTTCCTTATCAAGCATAATATAG
ACCTTAATGTTTTAAGTGAGATCTTAAGCGATCCTAAAATAGAGGTAGTCCAGAACGATGTTGAAGACGTCTATTATGCT
TTAACCAAGAAAAGTGAGATAAAAGGCTATGACGATTTTAACGACTTCTTAATTTTGTCTACAGCTATAACGCAAAAGTT
ACCTTTACTTACATTCGATAAAAAGTTGAAGAAAAAGATGGGGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8832.13 Da
• Isoelectric Point: 6.3353

>AT40172 WP_236752115.1 NZ_AP025245:62627-62866 [Acidianus sp. HS-5]
MEEVKVTRNYQITIPYNVRERLGIKVGDNLVVYLEGDKIIIQKKRGNIASLNLTLGKKITDEEINEAINEAGEKIGSSS

Nucleotide

• Length: 240 bp

>AT40172 NZ_AP025245:62627-62866 [Acidianus sp. HS-5]
ATGGAAGAAGTTAAAGTGACTAGAAATTATCAGATCACAATTCCGTACAATGTGAGAGAGAGACTAGGTATAAAGGTGGG
CGATAACTTAGTAGTTTACCTTGAGGGAGATAAAATAATCATACAGAAAAAAAGAGGCAATATTGCTTCTCTTAACTTGA
CACTAGGCAAGAAAATAACTGATGAGGAAATTAATGAAGCGATAAATGAGGCTGGAGAAAAAATTGGAAGCAGTAGTTGA