Detailed information of TA system TA040063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2801021..2801622
• Replicon: chromosome
• Accession: NZ_AP025193
• Organism: Comamonas thiooxydans strain NR4028

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Cthiooxydans_RS12845
• Protein ID: WP_003055100.1
• Coordinates: 2801021..2801407 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7WTR2
• Locus tag: Cthiooxydans_RS12850
• Protein ID: WP_003055097.1
• Coordinates: 2801407..2801622 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Cthiooxydans_RS12820 (Cthiooxydans_24720)	2796431..2796796	+	366	WP_001277463.1	mercury resistance co-regulator MerD	-
Cthiooxydans_RS12825 (Cthiooxydans_24730)	2796793..2797029	+	237	WP_003055146.1	broad-spectrum mercury transporter MerE	-
Cthiooxydans_RS12830 (Cthiooxydans_24740)	2797095..2797307	+	213	WP_000993245.1	DUF3330 domain-containing protein	-
Cthiooxydans_RS12835 (Cthiooxydans_24750)	2797437..2797997	+	561	WP_003055107.1	recombinase family protein	-
Cthiooxydans_RS12840	2798000..2800968	+	2969	Protein_2486	Tn3 family transposase	-
Cthiooxydans_RS12845 (Cthiooxydans_24780)	2801021..2801407	-	387	WP_003055100.1	type II toxin-antitoxin system VapC family toxin	Toxin
Cthiooxydans_RS12850 (Cthiooxydans_24790)	2801407..2801622	-	216	WP_003055097.1	DUF2191 domain-containing protein	Antitoxin
Cthiooxydans_RS12855 (Cthiooxydans_24800)	2801708..2802532	+	825	WP_003055094.1	SprT-like domain-containing protein	-
Cthiooxydans_RS12860 (Cthiooxydans_24810)	2802847..2805351	-	2505	WP_080554276.1	heavy metal translocating P-type ATPase	-
Cthiooxydans_RS12865 (Cthiooxydans_24820)	2805636..2806562	-	927	WP_003071641.1	copper homeostasis membrane protein CopD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (11-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13816.18 Da
• Isoelectric Point: 5.9593

>T40063 WP_003055100.1 NZ_AP025193:c2801407-2801021 [Comamonas thiooxydans]
MPVSVLVDTSVWVRHFREADIHLQELLLQDSVLMHPMVHAELACGTPPAPRADTLAALAMLRPSQEASIPETLAFLEKNK
LFGKGCGLVDLSLLASTLITPGALLWTADRRLAELAAQLGVAYAIPTH

Nucleotide

• Length: 387 bp

>T40063 NZ_AP025193:c2801407-2801021 [Comamonas thiooxydans]
ATGCCGGTGTCCGTTCTAGTCGATACATCGGTGTGGGTGAGGCATTTTCGGGAGGCCGACATTCACCTGCAGGAGCTCCT
GTTGCAAGACAGCGTGCTGATGCATCCCATGGTGCATGCAGAGTTGGCCTGCGGCACGCCGCCAGCGCCGCGTGCCGATA
CGCTCGCAGCCCTGGCCATGCTGCGGCCGAGCCAGGAAGCTTCAATTCCCGAGACTTTGGCCTTCCTTGAGAAGAACAAG
CTATTCGGTAAGGGCTGCGGCTTGGTCGATCTATCGCTGCTCGCCTCGACGCTAATCACACCAGGCGCTTTGCTTTGGAC
TGCAGATCGACGCCTTGCTGAGTTGGCCGCTCAGCTTGGCGTGGCCTATGCCATACCAACGCATTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7448.46 Da
• Isoelectric Point: 6.2846

>AT40063 WP_003055097.1 NZ_AP025193:c2801622-2801407 [Comamonas thiooxydans]
MRSTFTIDDEVVNRARAVAAPGIAVPELVRLALETFTRVEAGKRLAALGGSAPDMPDVPRRGSAAEAEGSR

Nucleotide

• Length: 216 bp

>AT40063 NZ_AP025193:c2801622-2801407 [Comamonas thiooxydans]
ATGAGATCGACCTTTACGATCGACGACGAAGTCGTGAACCGTGCCCGCGCTGTTGCAGCGCCCGGTATTGCTGTGCCTGA
ACTGGTACGCCTGGCACTTGAGACCTTCACTCGCGTCGAGGCAGGCAAGCGCTTGGCTGCCCTGGGCGGAAGCGCGCCAG
ACATGCCTGACGTGCCACGCCGTGGCAGCGCGGCAGAGGCAGAAGGCTCTCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A4Y8TDR8