Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 16635..16899 Replicon plasmid p538_L
Accession NZ_AP025182
Organism Escherichia coli strain JP538

Toxin (Protein)


Gene name pndA Uniprot ID U9Z417
Locus tag R5G_RS25270 Protein ID WP_001387489.1
Coordinates 16747..16899 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 16635..16695 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R5G_RS25255 (12737) 12737..13807 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
R5G_RS25260 (13826) 13826..15034 - 1209 WP_000121263.1 IncI1-type conjugal transfer protein TrbA -
R5G_RS25265 (15341) 15341..16120 - 780 WP_275450201.1 protein FinQ -
- (16635) 16635..16695 - 61 NuclAT_0 - Antitoxin
- (16635) 16635..16695 - 61 NuclAT_0 - Antitoxin
- (16635) 16635..16695 - 61 NuclAT_0 - Antitoxin
- (16635) 16635..16695 - 61 NuclAT_0 - Antitoxin
R5G_RS25270 (16747) 16747..16899 + 153 WP_001387489.1 Hok/Gef family protein Toxin
R5G_RS25275 (16971) 16971..17222 - 252 WP_001291964.1 hypothetical protein -
R5G_RS25870 (17965) 17965..18060 + 96 WP_000609148.1 DinQ-like type I toxin DqlB -
R5G_RS25280 (18125) 18125..18367 - 243 WP_000504400.1 hypothetical protein -
R5G_RS25285 (18486) 18486..18695 - 210 WP_001140543.1 hemolysin expression modulator Hha -
R5G_RS25290 (18793) 18793..19455 - 663 WP_124983586.1 plasmid IncI1-type surface exclusion protein ExcA -
R5G_RS25295 (19526) 19526..21694 - 2169 WP_000698368.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 - 1..89026 89026


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5761.10 Da        Isoelectric Point: 8.7948

>T40054 WP_001387489.1 NZ_AP025182:16747-16899 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T40054 NZ_AP025182:16747-16899 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 61 bp

>AT40054 NZ_AP025182:c16695-16635 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z417

Antitoxin

Download structure file

References