Detailed information of TA system TA039884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 199019..199692
• Replicon: plasmid pTT27
• Accession: NZ_AP024985
• Organism: Thermus thermophilus strain JCM10941

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q53W55
• Locus tag: LDO79_RS11045
• Protein ID: WP_011229190.1
• Coordinates: 199019..199279 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q53W54
• Locus tag: LDO79_RS11050
• Protein ID: WP_011229189.1
• Coordinates: 199300..199692 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO79_RS11025 (JCM10941_21510)	195400..196569	+	1170	WP_011229191.1	cytochrome P450	-
LDO79_RS11030 (JCM10941_21520)	196566..197264	+	699	WP_011174499.1	lycopene cyclase domain-containing protein	-
LDO79_RS11035 (JCM10941_21530)	197269..197946	+	678	WP_011174498.1	lysophospholipid acyltransferase family protein	-
LDO79_RS11040 (JCM10941_21540)	197943..198965	+	1023	WP_011174497.1	glycosyltransferase	-
LDO79_RS11045 (JCM10941_21550)	199019..199279	+	261	WP_011229190.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
LDO79_RS11050 (JCM10941_21560)	199300..199692	+	393	WP_011229189.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
LDO79_RS11055 (JCM10941_21570)	199790..201358	+	1569	WP_011229188.1	phytoene desaturase family protein	-
LDO79_RS11060 (JCM10941_21580)	201355..202353	+	999	WP_011229187.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
LDO79_RS11065 (JCM10941_21590)	202363..202755	+	393	WP_011174492.1	nucleotidyltransferase domain-containing protein	-
LDO79_RS11070 (JCM10941_21600)	202786..203733	-	948	WP_011229186.1	DUF523 and DUF1722 domain-containing protein	-
LDO79_RS11075 (JCM10941_21610)	203730..204518	-	789	WP_011229185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256991	256991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Antitoxin

• PIN domain (2-121)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9894.40 Da
• Isoelectric Point: 8.5953

>T39884 WP_011229190.1 NZ_AP024985:199019-199279 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>T39884 NZ_AP024985:199019-199279 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14264.67 Da
• Isoelectric Point: 10.6447

>AT39884 WP_011229189.1 NZ_AP024985:199300-199692 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATTLRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>AT39884 NZ_AP024985:199300-199692 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAAGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTAGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGACCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Structures

Toxin

• AlphaFold DB: Q53W55

Antitoxin

• AlphaFold DB: Q53W54