Detailed information of TA system TA039748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1654406..1654959
• Replicon: chromosome
• Accession: NZ_AP024847
• Organism: Serratia marcescens strain 2020-O-9

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A2V4FZ31
• Locus tag: LDN81_RS08075
• Protein ID: WP_019454426.1
• Coordinates: 1654645..1654959 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: LDN81_RS08070
• Protein ID: WP_039566839.1
• Coordinates: 1654406..1654642 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN81_RS08055 (SMGES_15780)	1650873..1652408	+	1536	WP_041034181.1	glutathione ABC transporter substrate-binding protein GsiB	-
LDN81_RS08060 (SMGES_15790)	1652461..1653381	+	921	WP_033637716.1	glutathione ABC transporter permease GsiC	-
LDN81_RS08065 (SMGES_15800)	1653391..1654299	+	909	WP_049233469.1	glutathione ABC transporter permease GsiD	-
LDN81_RS08070 (SMGES_15810)	1654406..1654642	+	237	WP_039566839.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LDN81_RS08075 (SMGES_15820)	1654645..1654959	+	315	WP_019454426.1	CcdB family protein	Toxin
LDN81_RS08080 (SMGES_15830)	1654999..1655841	-	843	WP_041034184.1	S-formylglutathione hydrolase	-
LDN81_RS08085 (SMGES_15840)	1655856..1656980	-	1125	WP_041034186.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
LDN81_RS08090 (SMGES_15850)	1657011..1657931	-	921	WP_033654969.1	LysR family transcriptional regulator	-
LDN81_RS08095 (SMGES_15860)	1658037..1659194	+	1158	WP_033654978.1	YbfB/YjiJ family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11745.68 Da
• Isoelectric Point: 5.0488

>T39748 WP_019454426.1 NZ_AP024847:1654645-1654959 [Serratia marcescens]
MQFTVYANRGNSAVYPLLLDVTSDIIGQLNRRVVIPLLPVEKYPGSTRPERLIPLIRLIDDNEYAVMTYEMASIPVRALG
AEFCDVSQYRTRIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T39748 NZ_AP024847:1654645-1654959 [Serratia marcescens]
ATGCAATTTACGGTGTATGCGAATAGGGGGAACAGCGCGGTTTATCCCCTGCTGCTTGATGTGACGAGCGATATCATTGG
GCAATTAAATCGTCGAGTTGTGATCCCTTTGTTGCCCGTTGAAAAATACCCTGGGAGTACGCGACCGGAACGCTTGATCC
CGTTGATCAGATTGATTGATGACAACGAGTACGCCGTGATGACATATGAAATGGCCAGCATCCCGGTTCGGGCTTTGGGG
GCCGAGTTTTGTGATGTTTCCCAGTATCGGACCAGGATAAAAGCCGCCATCGACTTTCTTCTTGACGGTATTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9068.10 Da
• Isoelectric Point: 5.7070

>AT39748 WP_039566839.1 NZ_AP024847:1654406-1654642 [Serratia marcescens]
MPVKQRTTQSVTMTVERSLLSRAREAGINLSATLTAALDAELRQHERIKWQEENREALEALNRFNDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT39748 NZ_AP024847:1654406-1654642 [Serratia marcescens]
ATGCCAGTAAAACAACGAACGACGCAAAGTGTGACCATGACGGTGGAACGCAGTTTGTTGAGCCGTGCGCGTGAGGCGGG
GATTAATCTCAGCGCGACGCTAACTGCTGCTTTAGATGCTGAGTTACGTCAGCATGAGAGGATAAAATGGCAGGAAGAAA
ACCGAGAGGCGCTTGAAGCGCTTAACCGATTTAATGATGAGCATGGTTGCTTTAGCGACGAATACAGGACGTTTTAG

Structures

Toxin

• AlphaFold DB: A0A2V4FZ31