Detailed information of TA system TA039521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 60089..60732
• Replicon: plasmid IncN-R
• Accession: NZ_AP024764
• Organism: Klebsiella pneumoniae strain TUM12130

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: K6J83_RS26700
• Protein ID: WP_001044770.1
• Coordinates: 60089..60505 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: K6J83_RS26705
• Protein ID: WP_001261282.1
• Coordinates: 60502..60732 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J83_RS26665 (TUM12130_51390)	55631..55867	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
K6J83_RS26670 (TUM12130_51400)	55864..56226	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
K6J83_RS26675 (TUM12130_51410)	56244..57938	-	1695	WP_000105636.1	mercury(II) reductase	-
K6J83_RS26680 (TUM12130_51420)	57990..58412	-	423	WP_001340589.1	organomercurial transporter MerC	-
K6J83_RS26685	58448..58558	-	111	Protein_65	mercuric transport protein periplasmic component	-
K6J83_RS26695 (TUM12130_51450)	59326..60015	-	690	Protein_67	AAA family ATPase	-
K6J83_RS26700 (TUM12130_51460)	60089..60505	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J83_RS26705 (TUM12130_51470)	60502..60732	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6J83_RS26710	60689..61150	+	462	WP_072202616.1	hypothetical protein	-
K6J83_RS26715 (TUM12130_51480)	61294..61644	+	351	WP_000493378.1	hypothetical protein	-
K6J83_RS26720 (TUM12130_51490)	61695..62438	+	744	WP_000129823.1	hypothetical protein	-
K6J83_RS26725 (TUM12130_51500)	62435..63211	+	777	WP_000015958.1	site-specific integrase	-
K6J83_RS27325 (TUM12130_51510)	63269..63448	-	180	Protein_74	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia	-	1..122843	122843
	-	inside	IScluster/Tn	aac(6')-Ib	-	58574..69050	10476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T39521 WP_001044770.1 NZ_AP024764:c60505-60089 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T39521 NZ_AP024764:c60505-60089 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT39521 WP_001261282.1 NZ_AP024764:c60732-60502 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT39521 NZ_AP024764:c60732-60502 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3