Detailed information of TA system TA039423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 1494752..1495266
• Replicon: chromosome
• Accession: NZ_AP024745
• Organism: Actinoplanes sp. L3-i22

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L3i22_RS06985
• Protein ID: WP_221326158.1
• Coordinates: 1494752..1495012 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: L3i22_RS06990
• Protein ID: WP_221326159.1
• Coordinates: 1495009..1495266 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3i22_RS06970 (L3i22_013870)	1492042..1492512	+	471	WP_221326157.1	helix-turn-helix domain-containing protein	-
L3i22_RS06975 (L3i22_013880)	1492522..1493088	+	567	WP_255658011.1	PIN domain-containing protein	-
L3i22_RS06980 (L3i22_013900)	1493508..1494697	+	1190	WP_221329827.1	IS3 family transposase	-
L3i22_RS06985 (L3i22_013910)	1494752..1495012	-	261	WP_221326158.1	Txe/YoeB family addiction module toxin	Toxin
L3i22_RS06990 (L3i22_013920)	1495009..1495266	-	258	WP_221326159.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
L3i22_RS06995	1496453..1496956	+	504	WP_221326160.1	DUF1707 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-70)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10210.54 Da
• Isoelectric Point: 7.2750

>T39423 WP_221326158.1 NZ_AP024745:c1495012-1494752 [Actinoplanes sp. L3-i22]
VKLSWTDHGWDDYLYWQTQDRKTLKRINALIADIKRDPDGPGIGKPEILRNNLAGLRSRRIDDEHRMVYAVEPNEITIIS
CRYHYE

Nucleotide

• Length: 261 bp

>T39423 NZ_AP024745:c1495012-1494752 [Actinoplanes sp. L3-i22]
GTGAAGCTCAGCTGGACCGATCACGGCTGGGACGACTACCTGTACTGGCAGACCCAGGACCGCAAGACCCTCAAGCGCAT
CAACGCGCTGATCGCCGACATCAAACGGGATCCCGACGGACCCGGCATCGGGAAACCGGAGATCCTCCGGAACAACCTCG
CCGGACTCCGGTCCCGGCGCATCGACGACGAACACCGCATGGTGTACGCCGTCGAACCCAACGAGATCACCATCATCTCC
TGCCGATATCACTACGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.70 Da
• Isoelectric Point: 4.8723

>AT39423 WP_221326159.1 NZ_AP024745:c1495266-1495009 [Actinoplanes sp. L3-i22]
VTAAISASEARKTLFPLIERVNEDHTPIEIVSKRGNAVLVSKDDWDSIMETNYLLRSPANAKRLAESVEQWRAGRATERE
FGPEE

Nucleotide

• Length: 258 bp

>AT39423 NZ_AP024745:c1495266-1495009 [Actinoplanes sp. L3-i22]
GTGACTGCCGCGATCAGCGCCAGCGAGGCACGTAAGACCCTCTTCCCGCTCATCGAACGGGTCAACGAAGACCACACCCC
GATCGAGATCGTGTCCAAACGGGGCAACGCAGTCCTGGTGAGCAAGGACGACTGGGATTCGATCATGGAGACCAACTACC
TGCTTCGCTCCCCGGCGAACGCGAAGCGCCTCGCCGAAAGCGTCGAGCAGTGGCGGGCCGGCCGAGCCACCGAGCGCGAG
TTCGGCCCCGAGGAGTGA