Detailed information of TA system TA038901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 23700..24343
• Replicon: plasmid pKLVA-1
• Accession: NZ_AP024593
• Organism: Klebsiella variicola strain JCM 12419

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KI238_RS26955
• Protein ID: WP_119867232.1
• Coordinates: 23927..24343 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: KI238_RS26950
• Protein ID: WP_119867231.1
• Coordinates: 23700..23930 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI238_RS26925 (KLVA_52160)	19445..19930	-	486	WP_119867226.1	TIR domain-containing protein	-
KI238_RS26930 (KLVA_52170)	19942..20469	-	528	WP_119867227.1	toll/interleukin-1 receptor domain-containing protein	-
KI238_RS26935 (KLVA_52180)	21023..21253	+	231	WP_119867228.1	type II toxin-antitoxin system VapB family antitoxin	-
KI238_RS26940 (KLVA_52190)	21250..21666	+	417	WP_119867229.1	type II toxin-antitoxin system VapC family toxin	-
KI238_RS26945 (KLVA_52210)	22170..23180	-	1011	WP_123797896.1	hypothetical protein	-
KI238_RS26950 (KLVA_52220)	23700..23930	+	231	WP_119867231.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KI238_RS26955 (KLVA_52230)	23927..24343	+	417	WP_119867232.1	type II toxin-antitoxin system VapC family toxin	Toxin
KI238_RS26960 (KLVA_52240)	24485..24703	+	219	WP_119867233.1	hypothetical protein	-
KI238_RS26965 (KLVA_52250)	24782..25108	+	327	WP_123797897.1	hypothetical protein	-
KI238_RS26970 (KLVA_52260)	25348..25605	+	258	WP_119867235.1	hypothetical protein	-
KI238_RS26975 (KLVA_52270)	25605..26171	+	567	WP_119867236.1	hypothetical protein	-
KI238_RS26980 (KLVA_52280)	26202..26693	+	492	WP_119867237.1	DNA-binding protein	-
KI238_RS26985 (KLVA_52290)	26717..27229	-	513	WP_123797898.1	hypothetical protein	-
KI238_RS26990 (KLVA_52300)	27852..28637	+	786	WP_123797899.1	hypothetical protein	-
KI238_RS26995 (KLVA_52310)	28800..28997	-	198	WP_123797900.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..163252	163252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15038.58 Da
• Isoelectric Point: 9.2957

>T38901 WP_119867232.1 NZ_AP024593:23927-24343 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAMLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T38901 NZ_AP024593:23927-24343 [Klebsiella variicola]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTACTGAAGCGCCT
GGAGCAGGCGATGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGCGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8663.71 Da
• Isoelectric Point: 4.4563

>AT38901 WP_119867231.1 NZ_AP024593:23700-23930 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLGRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT38901 NZ_AP024593:23700-23930 [Klebsiella variicola]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
GCAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA