Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4271809..4272428 Replicon chromosome
Accession NZ_AP024592
Organism Klebsiella variicola strain JCM 12419

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KI238_RS20715 Protein ID WP_002892050.1
Coordinates 4272210..4272428 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag KI238_RS20710 Protein ID WP_008805436.1
Coordinates 4271809..4272183 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI238_RS20700 (KLVA_40310) 4266964..4268157 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KI238_RS20705 (KLVA_40320) 4268180..4271326 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
KI238_RS20710 (KLVA_40330) 4271809..4272183 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
KI238_RS20715 (KLVA_40340) 4272210..4272428 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KI238_RS20720 (KLVA_40350) 4272587..4273153 + 567 WP_022066286.1 maltose O-acetyltransferase -
KI238_RS20725 4273125..4273250 - 126 Protein_4086 hypothetical protein -
KI238_RS20730 (KLVA_40360) 4273290..4273760 + 471 WP_008805434.1 YlaC family protein -
KI238_RS20735 (KLVA_40370) 4273729..4275186 - 1458 WP_022066287.1 PLP-dependent aminotransferase family protein -
KI238_RS20740 (KLVA_40380) 4275287..4275985 + 699 WP_119867109.1 GNAT family protein -
KI238_RS20745 (KLVA_40390) 4275982..4276122 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KI238_RS20750 (KLVA_40400) 4276122..4276385 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T38894 WP_002892050.1 NZ_AP024592:4272210-4272428 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T38894 NZ_AP024592:4272210-4272428 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT38894 WP_008805436.1 NZ_AP024592:4271809-4272183 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT38894 NZ_AP024592:4271809-4272183 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References