Detailed information of TA system TA038893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4139419..4140016
• Replicon: chromosome
• Accession: NZ_AP024592
• Organism: Klebsiella variicola strain JCM 12419

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: KI238_RS20095
• Protein ID: WP_012542526.1
• Coordinates: 4139699..4140016 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KI238_RS20090
• Protein ID: WP_012542525.1
• Coordinates: 4139419..4139706 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI238_RS20060 (KLVA_39050)	4135279..4135527	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
KI238_RS20065 (KLVA_39060)	4135544..4135885	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KI238_RS20070 (KLVA_39070)	4135916..4137031	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
KI238_RS20075 (KLVA_39080)	4137211..4137792	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
KI238_RS20080 (KLVA_39090)	4137792..4138160	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
KI238_RS20085 (KLVA_39100)	4138280..4138933	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
KI238_RS20090 (KLVA_39110)	4139419..4139706	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
KI238_RS20095 (KLVA_39120)	4139699..4140016	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI238_RS20100 (KLVA_39130)	4140201..4141244	-	1044	WP_119867102.1	DUF2157 domain-containing protein	-
KI238_RS20105 (KLVA_39140)	4141905..4142771	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
KI238_RS20110 (KLVA_39150)	4142880..4144307	+	1428	WP_049009040.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T38893 WP_012542526.1 NZ_AP024592:c4140016-4139699 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T38893 NZ_AP024592:c4140016-4139699 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGTTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCATCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT38893 WP_012542525.1 NZ_AP024592:c4139706-4139419 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT38893 NZ_AP024592:c4139706-4139419 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2