Detailed information of TA system TA038474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1502589..1503201
• Replicon: chromosome
• Accession: NZ_AP024487
• Organism: MAG: Metallosphaera javensis (ex Sakai et al. 2022) isolate AS-7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QVC96_RS08755
• Protein ID: WP_238026591.1
• Coordinates: 1502830..1503201 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: QVC96_RS08750
• Protein ID: WP_238026590.1
• Coordinates: 1502589..1502816 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QVC96_RS08715 (MjAS7_1832)	1497631..1498446	+	816	WP_287688944.1	pyrroline-5-carboxylate reductase	-
QVC96_RS08720 (MjAS7_1833)	1498443..1499069	-	627	WP_287688945.1	hypothetical protein	-
QVC96_RS08725 (MjAS7_1834)	1499056..1499688	-	633	WP_287688946.1	thymidylate kinase	-
QVC96_RS08730 (MjAS7_1835)	1499670..1500311	-	642	WP_238026586.1	dTMP kinase	-
QVC96_RS08735 (MjAS7_1836)	1500311..1501573	-	1263	WP_238026587.1	Ppx/GppA phosphatase family protein	-
QVC96_RS08740 (MjAS7_1837)	1501574..1502044	-	471	WP_287688947.1	phosphohistidine phosphatase SixA	-
QVC96_RS08745 (MjAS7_1838)	1502144..1502539	+	396	WP_287688948.1	hypothetical protein	-
QVC96_RS08750 (MjAS7_1839)	1502589..1502816	+	228	WP_238026590.1	VapB-type antitoxin	Antitoxin
QVC96_RS08755 (MjAS7_1840)	1502830..1503201	+	372	WP_238026591.1	type II toxin-antitoxin system VapC family toxin	Toxin
QVC96_RS08760 (MjAS7_1841)	1503268..1504068	-	801	WP_238026592.1	amidohydrolase family protein	-
QVC96_RS08765 (MjAS7_1842)	1504171..1504962	-	792	WP_287688949.1	AAA family ATPase	-
QVC96_RS08770 (MjAS7_1844)	1505536..1506266	-	731	Protein_1716	IS1 family transposase	-
QVC96_RS08775 (MjAS7_1845)	1506372..1506632	+	261	WP_287688950.1	AAA family ATPase	-
QVC96_RS08780 (MjAS7_1846)	1506716..1507609	-	894	Protein_1718	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1470032..1504710	34678

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Putative antitoxin VapB3-like (4-75)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13815.13 Da
• Isoelectric Point: 4.7904

>T38474 WP_238026591.1 NZ_AP024487:1502830-1503201 [Metallosphaera javensis (ex Sakai et al. 2022)]
VKYFSKEKGWEVVERVFLQGDLMTLDLALKEVANALWKKVLREEMTLEDSLKLINTLKEGVIVEIVPGNAYLEEGFRIAV
RQKSSIYDSLFIALAKSVGGKLITSDLMQRNAGINEGIEVEVI

Nucleotide

• Length: 372 bp

>T38474 NZ_AP024487:1502830-1503201 [Metallosphaera javensis (ex Sakai et al. 2022)]
GTGAAGTACTTCTCAAAGGAAAAGGGATGGGAGGTTGTAGAAAGGGTATTTCTTCAAGGGGACCTGATGACCCTTGATCT
GGCACTCAAGGAGGTGGCCAACGCTCTCTGGAAGAAAGTTCTAAGAGAGGAGATGACACTAGAGGACTCCCTTAAGCTGA
TCAATACGTTAAAGGAAGGGGTGATAGTGGAGATAGTGCCAGGAAACGCTTACCTAGAGGAAGGTTTCAGAATTGCTGTA
AGGCAGAAGTCCTCAATTTACGATTCGCTATTCATTGCCCTGGCAAAGTCAGTCGGAGGTAAGTTAATCACCTCGGATCT
CATGCAGAGAAACGCCGGAATTAATGAGGGAATAGAGGTAGAGGTGATATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 9014.25 Da
• Isoelectric Point: 7.2055

>AT38474 WP_238026590.1 NZ_AP024487:1502589-1502816 [Metallosphaera javensis (ex Sakai et al. 2022)]
MSTKVISVRIRKEIVEELEKHGINVNDEVRKAIEELYKGMKKREYLELWRETLQRVEPSEEGFSVRSVRQDRDSH

Nucleotide

• Length: 228 bp

>AT38474 NZ_AP024487:1502589-1502816 [Metallosphaera javensis (ex Sakai et al. 2022)]
ATGTCTACAAAAGTCATAAGTGTTAGGATAAGGAAGGAGATCGTTGAGGAACTAGAGAAGCATGGTATCAACGTGAACGA
TGAGGTAAGGAAAGCCATTGAGGAACTTTACAAGGGGATGAAGAAAAGGGAATACCTAGAACTCTGGAGGGAGACTCTTC
AACGCGTTGAACCCAGTGAAGAGGGTTTCTCGGTTAGGAGCGTGAGGCAGGATCGCGATAGTCATTGA