Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5161458..5162083 Replicon chromosome
Accession NZ_AP024173
Organism Klebsiella pneumoniae strain KSB1_9A

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JGR95_RS25295 Protein ID WP_002882817.1
Coordinates 5161458..5161841 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JGR95_RS25300 Protein ID WP_004150355.1
Coordinates 5161841..5162083 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGR95_RS25280 5158824..5159726 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JGR95_RS25285 5159723..5160358 + 636 WP_201357899.1 formate dehydrogenase cytochrome b556 subunit -
JGR95_RS25290 5160355..5161284 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JGR95_RS25295 5161458..5161841 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JGR95_RS25300 5161841..5162083 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JGR95_RS25305 5162288..5163194 + 907 Protein_4943 alpha/beta hydrolase -
JGR95_RS25310 5163209..5164150 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JGR95_RS25315 5164195..5164632 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JGR95_RS25320 5164629..5165489 - 861 WP_004146232.1 virulence factor BrkB family protein -
JGR95_RS25325 5165483..5166082 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T38102 WP_002882817.1 NZ_AP024173:c5161841-5161458 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T38102 NZ_AP024173:c5161841-5161458 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT38102 WP_004150355.1 NZ_AP024173:c5162083-5161841 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT38102 NZ_AP024173:c5162083-5161841 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References