Detailed information of TA system TA037964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4601783..4602620
• Replicon: chromosome
• Accession: NZ_AP024123
• Organism: Escherichia coli strain KS-NP019

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FFG67_RS22225
• Protein ID: WP_000227784.1
• Coordinates: 4601783..4602325 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FFG67_RS22230
• Protein ID: WP_001297137.1
• Coordinates: 4602309..4602620 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFG67_RS22205 (4597322)	4597322..4598233	-	912	WP_000705849.1	2-dehydropantoate 2-reductase	-
FFG67_RS22210 (4598401)	4598401..4598892	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
FFG67_RS22215 (4599020)	4599020..4600384	-	1365	WP_047083808.1	MFS transporter	-
FFG67_RS22220 (4600792)	4600792..4601727	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
FFG67_RS22225 (4601783)	4601783..4602325	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FFG67_RS22230 (4602309)	4602309..4602620	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FFG67_RS22235 (4602805)	4602805..4603695	-	891	WP_000971336.1	heme o synthase	-
FFG67_RS22240 (4603707)	4603707..4604036	-	330	WP_100620279.1	cytochrome o ubiquinol oxidase subunit IV	-
FFG67_RS22245 (4604036)	4604036..4604650	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FFG67_RS22250 (4604640)	4604640..4606631	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FFG67_RS22255 (4606653)	4606653..4607600	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T37964 WP_000227784.1 NZ_AP024123:c4602325-4601783 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T37964 NZ_AP024123:c4602325-4601783 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT37964 WP_001297137.1 NZ_AP024123:c4602620-4602309 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT37964 NZ_AP024123:c4602620-4602309 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3