Detailed information of TA system TA037869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1262641..1263368
• Replicon: chromosome
• Accession: NZ_AP024112
• Organism: Escherichia coli strain ECSC054

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: FE985_RS06030
• Protein ID: WP_000547555.1
• Coordinates: 1262641..1262952 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FE985_RS06035
• Protein ID: WP_000126294.1
• Coordinates: 1262949..1263368 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE985_RS06005 (1258576)	1258576..1260285	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
FE985_RS06010 (1260295)	1260295..1260837	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
FE985_RS06015 (1260837)	1260837..1261604	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FE985_RS06020 (1261601)	1261601..1262011	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
FE985_RS06025 (1262004)	1262004..1262474	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
FE985_RS06030 (1262641)	1262641..1262952	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
FE985_RS06035 (1262949)	1262949..1263368	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FE985_RS06040 (1263447)	1263447..1264871	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
FE985_RS06045 (1264880)	1264880..1266337	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FE985_RS06050 (1266597)	1266597..1267607	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
FE985_RS06055 (1267756)	1267756..1268283	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T37869 WP_000547555.1 NZ_AP024112:1262641-1262952 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T37869 NZ_AP024112:1262641-1262952 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT37869 WP_000126294.1 NZ_AP024112:1262949-1263368 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT37869 NZ_AP024112:1262949-1263368 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2