Detailed information of TA system TA037823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 45254..45897
• Replicon: plasmid pM941-b
• Accession: NZ_AP023456
• Organism: Klebsiella pneumoniae strain M941

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JLF44_RS28180
• Protein ID: WP_001044770.1
• Coordinates: 45481..45897 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JLF44_RS28175
• Protein ID: WP_001261282.1
• Coordinates: 45254..45484 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF44_RS28140	40826..41692	-	867	WP_004118283.1	replication initiation protein	-
JLF44_RS28145	42227..42331	+	105	WP_032409716.1	hypothetical protein	-
JLF44_RS28150	42460..42717	+	258	WP_000764642.1	hypothetical protein	-
JLF44_RS28155	42775..43551	-	777	WP_000015958.1	site-specific integrase	-
JLF44_RS28160	43548..44291	-	744	WP_000129823.1	hypothetical protein	-
JLF44_RS28165	44342..44692	-	351	WP_000493378.1	hypothetical protein	-
JLF44_RS28170	44836..45297	-	462	WP_072202616.1	hypothetical protein	-
JLF44_RS28175	45254..45484	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JLF44_RS28180	45481..45897	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF44_RS28185	45971..46681	+	711	WP_004152334.1	ATP-binding protein	-
JLF44_RS28190	46715..47412	+	698	Protein_62	IS1 family transposase	-
JLF44_RS28195	47429..47620	-	192	Protein_63	nucleotidyltransferase domain-containing protein	-
JLF44_RS28200	47690..48244	-	555	WP_063840280.1	AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase	-
JLF44_RS28205	48478..48972	-	495	Protein_65	recombinase family protein	-
JLF44_RS28210	49033..49739	+	707	Protein_66	IS6 family transposase	-
JLF44_RS28215	50171..50877	-	707	Protein_67	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	qnrB9 / blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib / blaSHV-182	-	1..56841	56841

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T37823 WP_001044770.1 NZ_AP023456:45481-45897 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T37823 NZ_AP023456:45481-45897 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT37823 WP_001261282.1 NZ_AP023456:45254-45484 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT37823 NZ_AP023456:45254-45484 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3