Detailed information of TA system TA037727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2399317..2399955
• Replicon: chromosome
• Accession: NZ_AP023433
• Organism: Escherichia coli strain M719

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: M2043_RS11595
• Protein ID: WP_001447010.1
• Coordinates: 2399317..2399493 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: M2043_RS11600
• Protein ID: WP_001270286.1
• Coordinates: 2399539..2399955 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2043_RS11575 (2394936)	2394936..2396150	-	1215	WP_001326689.1	BenE family transporter YdcO	-
M2043_RS11580 (2396203)	2396203..2396739	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
M2043_RS11585 (2396812)	2396812..2398773	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
M2043_RS11590 (2398865)	2398865..2399095	-	231	WP_000494244.1	YncJ family protein	-
M2043_RS11595 (2399317)	2399317..2399493	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
M2043_RS11600 (2399539)	2399539..2399955	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
M2043_RS11605 (2400034)	2400034..2401440	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
M2043_RS11610 (2401674)	2401674..2402819	+	1146	WP_000047428.1	ABC transporter substrate-binding protein	-
M2043_RS11615 (2402837)	2402837..2403850	+	1014	WP_000220395.1	ABC transporter ATP-binding protein	-
M2043_RS11620 (2403851)	2403851..2404792	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T37727 WP_001447010.1 NZ_AP023433:2399317-2399493 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T37727 NZ_AP023433:2399317-2399493 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT37727 WP_001270286.1 NZ_AP023433:2399539-2399955 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT37727 NZ_AP023433:2399539-2399955 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8