Detailed information of TA system TA037066

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53838..54077
• Replicon: plasmid pF070-NDM5
• Accession: NZ_AP023238
• Organism: Escherichia coli strain F070

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: FJI53_RS23675
• Protein ID: WP_023144756.1
• Coordinates: 53838..53972 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	54017..54077 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJI53_RS23650	49539..50396	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
FJI53_RS23655	50389..50463	-	75	WP_031943482.1	RepA leader peptide Tap	-
FJI53_RS23910	50460..50594	-	135	Protein_63	protein CopA/IncA	-
FJI53_RS23660	50825..52366	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
FJI53_RS23665	52381..53127	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
FJI53_RS23670	53287..53541	-	255	WP_000083850.1	replication regulatory protein RepA	-
FJI53_RS23675	53838..53972	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	54017..54077	+	61	NuclAT_0	-	Antitoxin
-	54017..54077	+	61	NuclAT_0	-	Antitoxin
-	54017..54077	+	61	NuclAT_0	-	Antitoxin
-	54017..54077	+	61	NuclAT_0	-	Antitoxin
FJI53_RS23680	54044..54330	-	287	Protein_68	DUF2726 domain-containing protein	-
FJI53_RS23685	54843..55055	-	213	WP_013023861.1	hypothetical protein	-
FJI53_RS23690	55186..55746	-	561	WP_000139341.1	fertility inhibition protein FinO	-
FJI53_RS23695	55801..56547	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
FJI53_RS23700	56567..58960	-	2394	Protein_72	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA2 / dfrA12 / blaNDM-5 / aadA5 / blaTEM-1B / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aac(6')-Ib-cr	-	1..93649	93649

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T37066 WP_023144756.1 NZ_AP023238:c53972-53838 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T37066 NZ_AP023238:c53972-53838 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT37066 NZ_AP023238:54017-54077 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin