Detailed information of TA system TA037021

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1081194..1082026
• Replicon: chromosome
• Accession: NZ_AP023235
• Organism: Escherichia coli strain YJ6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: HXS64_RS05135
• Protein ID: WP_000854765.1
• Coordinates: 1081194..1081568 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HXS64_RS05140
• Protein ID: WP_001295723.1
• Coordinates: 1081658..1082026 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS64_RS05115	1078986..1080206	+	1221	WP_001735713.1	capsular biosynthesis protein	-
HXS64_RS05120	1080229..1080465	-	237	WP_001735712.1	hypothetical protein	-
HXS64_RS05125	1080568..1080744	-	177	WP_000839288.1	DUF957 domain-containing protein	-
HXS64_RS05130	1080761..1081197	-	437	Protein_992	hypothetical protein	-
HXS64_RS05135	1081194..1081568	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
HXS64_RS05140	1081658..1082026	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HXS64_RS05145	1082189..1082410	-	222	WP_021574928.1	DUF987 domain-containing protein	-
HXS64_RS05150	1082473..1082950	-	478	Protein_996	RadC family protein	-
HXS64_RS05155	1082966..1083451	-	486	WP_001552107.1	antirestriction protein	-
HXS64_RS05160	1083506..1084324	-	819	WP_065898639.1	DUF945 domain-containing protein	-
HXS64_RS05165	1084479..1084637	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T37021 WP_000854765.1 NZ_AP023235:c1081568-1081194 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T37021 NZ_AP023235:c1081568-1081194 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT37021 WP_001295723.1 NZ_AP023235:c1082026-1081658 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT37021 NZ_AP023235:c1082026-1081658 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52