Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1884204..1884829 Replicon chromosome
Accession NZ_AP023205
Organism Escherichia coli strain TUM18781

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HUV20_RS08935 Protein ID WP_000911329.1
Coordinates 1884431..1884829 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HUV20_RS08930 Protein ID WP_000450524.1
Coordinates 1884204..1884431 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUV20_RS08905 1880006..1880476 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HUV20_RS08910 1880476..1881048 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HUV20_RS08915 1881194..1882072 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HUV20_RS08920 1882089..1883123 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HUV20_RS08925 1883336..1884049 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HUV20_RS08930 1884204..1884431 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HUV20_RS08935 1884431..1884829 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
HUV20_RS08940 1884976..1885839 + 864 WP_001267498.1 neutral zinc metallopeptidase -
HUV20_RS08945 1885854..1887869 + 2016 WP_053264617.1 tRNA cytosine(34) acetyltransferase TmcA -
HUV20_RS08950 1887943..1888641 + 699 WP_000679812.1 esterase -
HUV20_RS08955 1888722..1888922 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T36865 WP_000911329.1 NZ_AP023205:1884431-1884829 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T36865 NZ_AP023205:1884431-1884829 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT36865 WP_000450524.1 NZ_AP023205:1884204-1884431 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT36865 NZ_AP023205:1884204-1884431 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References