Detailed information of TA system TA036794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 636173..636972
• Replicon: chromosome
• Accession: NZ_AP023190
• Organism: Escherichia coli strain TUM18530

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A161R7C1
• Locus tag: HUV24_RS03030
• Protein ID: WP_000347278.1
• Coordinates: 636173..636637 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: HUV24_RS03035
• Protein ID: WP_001307405.1
• Coordinates: 636637..636972 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUV24_RS03000	631174..631608	-	435	WP_113419634.1	PTS sugar transporter subunit IIA	-
HUV24_RS03005	631626..632504	-	879	WP_021557244.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HUV24_RS03010	632494..633273	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HUV24_RS03015	633284..633757	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HUV24_RS03020	633780..635060	-	1281	WP_021557243.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HUV24_RS03025	635309..636118	+	810	WP_000072174.1	aga operon transcriptional regulator AgaR	-
HUV24_RS03030	636173..636637	-	465	WP_000347278.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HUV24_RS03035	636637..636972	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HUV24_RS03040	637121..638692	-	1572	WP_001273763.1	galactarate dehydratase	-
HUV24_RS03045	639067..640401	+	1335	WP_174402605.1	galactarate/glucarate/glycerate transporter GarP	-
HUV24_RS03050	640417..641187	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17866.28 Da
• Isoelectric Point: 9.6924

>T36794 WP_000347278.1 NZ_AP023190:c636637-636173 [Escherichia coli]
MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T36794 NZ_AP023190:c636637-636173 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATACTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT36794 WP_001307405.1 NZ_AP023190:c636972-636637 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT36794 NZ_AP023190:c636972-636637 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A161R7C1

Antitoxin

• AlphaFold DB: A0A0E0XVC7