Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2491966..2492941 Replicon chromosome
Accession NZ_AP022964
Organism Bacillus cereus strain J62

Toxin (Protein)


Gene name spoIISA Uniprot ID C3GJ28
Locus tag L6484_RS12695 Protein ID WP_003297560.1
Coordinates 2491966..2492703 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag L6484_RS12700 Protein ID WP_000588712.1
Coordinates 2492816..2492941 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6484_RS12665 (BCJMU62_2490) 2487042..2487752 + 711 WP_000787475.1 class I SAM-dependent methyltransferase -
L6484_RS12670 (BCJMU62_2491) 2487872..2488279 + 408 WP_000072479.1 VOC family protein -
L6484_RS12675 2488385..2488477 + 93 Protein_2427 methyltransferase -
L6484_RS12680 (BCJMU62_2493) 2488599..2490283 - 1685 Protein_2428 alpha-keto acid decarboxylase family protein -
L6484_RS12685 (BCJMU62_2494) 2490390..2490872 + 483 WP_088016051.1 MarR family winged helix-turn-helix transcriptional regulator -
L6484_RS12690 (BCJMU62_2495) 2491040..2491777 + 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
L6484_RS12695 (BCJMU62_2496) 2491966..2492703 + 738 WP_003297560.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
L6484_RS12700 (BCJMU62_2497) 2492816..2492941 + 126 WP_000588712.1 hypothetical protein Antitoxin
L6484_RS12705 (BCJMU62_2498) 2493019..2493195 + 177 WP_000808045.1 stage II sporulation protein SB -
L6484_RS12710 (BCJMU62_2499) 2493339..2494808 + 1470 WP_070171971.1 beta-Ala-His dipeptidase -
L6484_RS12715 (BCJMU62_2500) 2495043..2495513 - 471 WP_000670597.1 DUF5065 family protein -
L6484_RS12720 (BCJMU62_2501) 2495717..2496310 + 594 WP_000265892.1 SGNH/GDSL hydrolase family protein -
L6484_RS12725 (BCJMU62_2502) 2496532..2497065 + 534 WP_202294512.1 sigma-70 family RNA polymerase sigma factor -
L6484_RS12730 (BCJMU62_2503) 2497055..2497882 + 828 WP_070171973.1 anti-sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2430170..2500399 70229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28373.07 Da        Isoelectric Point: 8.2672

>T36655 WP_003297560.1 NZ_AP022964:2491966-2492703 [Bacillus cereus]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T36655 NZ_AP022964:2491966-2492703 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTTGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT36655 WP_000588712.1 NZ_AP022964:2492816-2492941 [Bacillus cereus]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT36655 NZ_AP022964:2492816-2492941 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9EK80

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References