Detailed information of TA system TA036644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2385513..2386488
• Replicon: chromosome
• Accession: NZ_AP022921
• Organism: Bacillus cereus strain J1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6479_RS12235
• Protein ID: WP_062923173.1
• Coordinates: 2385513..2386250 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L6479_RS12240
• Protein ID: WP_062923165.1
• Coordinates: 2386363..2386488 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6479_RS12210 (BCJMU01_2361)	2381007..2381708	+	702	WP_236788434.1	hypothetical protein	-
L6479_RS12215 (BCJMU01_2362)	2381836..2382720	+	885	WP_062923162.1	hypothetical protein	-
L6479_RS12220	2383029..2383831	-	803	Protein_2331	thiamine pyrophosphate-binding protein	-
L6479_RS12225 (BCJMU01_2365)	2383938..2384420	+	483	WP_062923163.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6479_RS12230 (BCJMU01_2366)	2384587..2385324	+	738	WP_062923164.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6479_RS12235 (BCJMU01_2367)	2385513..2386250	+	738	WP_062923173.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6479_RS12240 (BCJMU01_2368)	2386363..2386488	+	126	WP_062923165.1	hypothetical protein	Antitoxin
L6479_RS12245 (BCJMU01_2369)	2386564..2386740	+	177	WP_062923166.1	stage II sporulation protein SB	-
L6479_RS12250 (BCJMU01_2370)	2386759..2387148	-	390	WP_062923167.1	YxeA family protein	-
L6479_RS12255 (BCJMU01_2371)	2387365..2388834	+	1470	WP_062923168.1	beta-Ala-His dipeptidase	-
L6479_RS12260 (BCJMU01_2372)	2389416..2389886	-	471	WP_061530815.1	DUF5065 family protein	-
L6479_RS12265 (BCJMU01_2373)	2390340..2390999	+	660	WP_000876955.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.03 Da
• Isoelectric Point: 8.3034

>T36644 WP_062923173.1 NZ_AP022921:2385513-2386250 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYIKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKTVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T36644 NZ_AP022921:2385513-2386250 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGTTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAGTTGTACGAAGAGAAGAAGCAACGGATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATATAAAAAGCTTAAACGCATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGCTCACAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCGGCAAAAACTGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAATCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT36644 WP_062923165.1 NZ_AP022921:2386363-2386488 [Bacillus cereus]
VIKDYDNDFFKEENEDTTDFSLIKGDTLQQVEELEDELKRK

Nucleotide

• Length: 126 bp

>AT36644 NZ_AP022921:2386363-2386488 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGAAAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGATGAATTAAAAAGAAAATGA