Detailed information of TA system TA036538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4625605..4626207
• Replicon: chromosome
• Accession: NZ_AP022815
• Organism: Escherichia coli strain JE86-ST05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JL003_RS22755
• Protein ID: WP_000897305.1
• Coordinates: 4625605..4625916 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JL003_RS22760
• Protein ID: WP_000356397.1
• Coordinates: 4625917..4626207 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL003_RS22725	4620635..4621420	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
JL003_RS22730	4621519..4622118	+	600	WP_001295269.1	glucose-1-phosphatase	-
JL003_RS22735	4622112..4622984	+	873	WP_000920762.1	virulence factor BrkB family protein	-
JL003_RS22740	4622981..4623418	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JL003_RS22745	4623463..4624404	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
JL003_RS22750	4624468..4625376	-	909	WP_001162704.1	alpha/beta hydrolase	-
JL003_RS22755	4625605..4625916	+	312	WP_000897305.1	hypothetical protein	Toxin
JL003_RS22760	4625917..4626207	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JL003_RS22765	4626812..4627030	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
JL003_RS22770	4627249..4627491	+	243	WP_001086388.1	hypothetical protein	-
JL003_RS22775	4627821..4628750	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JL003_RS22780	4628747..4629382	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JL003_RS22785	4629379..4630281	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T36538 WP_000897305.1 NZ_AP022815:4625605-4625916 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T36538 NZ_AP022815:4625605-4625916 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTGAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT36538 WP_000356397.1 NZ_AP022815:4625917-4626207 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT36538 NZ_AP022815:4625917-4626207 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7