Detailed information of TA system TA036345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 186468..187099
• Replicon: plasmid pJCM12687
• Accession: NZ_AP022607
• Organism: Mycobacterium branderi strain JCM 12687

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N47_RS25810
• Protein ID: WP_083134305.1
• Coordinates: 186722..187099 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N47_RS25805
• Protein ID: WP_083134304.1
• Coordinates: 186468..186725 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N47_RS25785	181827..182642	-	816	WP_083134301.1	mycofactocin-coupled SDR family oxidoreductase	-
G6N47_RS25790	182682..183155	-	474	WP_083134331.1	PaaI family thioesterase	-
G6N47_RS25795	183310..184779	+	1470	WP_083134302.1	aldehyde dehydrogenase family protein	-
G6N47_RS25800	184776..186380	+	1605	WP_083134303.1	GMC family oxidoreductase N-terminal domain-containing protein	-
G6N47_RS25805	186468..186725	+	258	WP_083134304.1	ribbon-helix-helix domain-containing protein	Antitoxin
G6N47_RS25810	186722..187099	+	378	WP_083134305.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N47_RS25815	187353..187949	+	597	WP_083134306.1	TetR family transcriptional regulator	-
G6N47_RS25820	188097..188345	+	249	WP_083134307.1	hypothetical protein	-
G6N47_RS25825	188405..189058	+	654	WP_083134308.1	hypothetical protein	-
G6N47_RS25830	189177..189539	+	363	WP_083134309.1	hypothetical protein	-
G6N47_RS25835	189586..189999	-	414	WP_083134310.1	nuclear transport factor 2 family protein	-
G6N47_RS25840	190010..191614	-	1605	WP_083134311.1	AMP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	esxN / esxN	1..864257	864257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13699.57 Da
• Isoelectric Point: 4.4093

>T36345 WP_083134305.1 NZ_AP022607:186722-187099 [Mycobacterium branderi]
MKLIDTTIAVDHLRGEPAAVELLASLVDDGEDLAASELVRFELLAGTRDKEVRALEEFCSALQWANVTEDIARVGGQLAR
RYRKSHSGIGAVDYLIAATAIVIDADLLTTNVRHFPMFTELDAPY

Nucleotide

• Length: 378 bp

>T36345 NZ_AP022607:186722-187099 [Mycobacterium branderi]
ATGAAGCTCATTGACACCACTATCGCTGTGGATCACCTGCGGGGCGAGCCTGCTGCTGTCGAGTTGCTCGCGTCGCTGGT
TGACGACGGCGAAGACCTAGCGGCCAGCGAATTGGTCCGATTCGAGCTCCTTGCGGGTACCCGAGACAAGGAGGTCCGTG
CGCTTGAGGAGTTCTGCTCGGCCCTGCAATGGGCCAACGTGACCGAAGACATTGCCCGTGTCGGGGGACAACTCGCCCGT
CGGTACCGAAAGAGCCATAGCGGTATCGGTGCCGTCGACTACTTGATCGCCGCGACTGCGATCGTCATCGATGCCGACCT
GCTGACGACGAATGTGCGGCATTTTCCAATGTTTACCGAGCTGGACGCCCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9437.52 Da
• Isoelectric Point: 10.6555

>AT36345 WP_083134304.1 NZ_AP022607:186468-186725 [Mycobacterium branderi]
VRTQITLNDEEIELLDRATKASGASRSELIRRAIRITYGSRNKDDRTAALKRSAGSWRGRSFTGADYVDAVRGDLNERLS
QLGLA

Nucleotide

• Length: 258 bp

>AT36345 NZ_AP022607:186468-186725 [Mycobacterium branderi]
GTGCGGACGCAAATCACGCTGAATGATGAGGAGATCGAACTCCTAGATCGCGCCACAAAGGCAAGTGGCGCATCCAGGTC
AGAACTGATTCGTCGGGCAATTCGCATCACGTACGGAAGCCGCAACAAAGATGACCGGACCGCCGCGCTGAAGCGCAGCG
CCGGCTCCTGGCGCGGGCGGAGCTTCACTGGTGCTGATTACGTCGACGCCGTTCGCGGCGACCTTAACGAGCGGCTAAGC
CAACTCGGTCTGGCATGA