Detailed information of TA system TA036329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 105828..106516
• Replicon: chromosome
• Accession: NZ_AP022606
• Organism: Mycobacterium branderi strain JCM 12687

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N47_RS00730
• Protein ID: WP_083129751.1
• Coordinates: 105828..106265 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1X1RUF8
• Locus tag: G6N47_RS00735
• Protein ID: WP_062539743.1
• Coordinates: 106262..106516 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N47_RS00710	101968..103170	+	1203	WP_083129747.1	cytochrome P450	-
G6N47_RS00715	103150..104469	-	1320	WP_197945492.1	amidohydrolase family protein	-
G6N47_RS00720	104494..105048	-	555	WP_083129749.1	pyridoxamine 5'-phosphate oxidase family protein	-
G6N47_RS00725	105081..105800	+	720	WP_083129750.1	TetR/AcrR family transcriptional regulator	-
G6N47_RS00730	105828..106265	-	438	WP_083129751.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N47_RS00735	106262..106516	-	255	WP_062539743.1	ribbon-helix-helix protein, CopG family	Antitoxin
G6N47_RS00740	106583..107581	+	999	WP_083129752.1	acryloyl-CoA reductase	-
G6N47_RS00745	107578..108060	-	483	WP_083129753.1	hypothetical protein	-
G6N47_RS00750	108219..109310	+	1092	WP_083129754.1	homogentisate 1,2-dioxygenase	-
G6N47_RS00755	109311..110498	+	1188	WP_083129755.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-39)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15335.42 Da
• Isoelectric Point: 6.3704

>T36329 WP_083129751.1 NZ_AP022606:c106265-105828 [Mycobacterium branderi]
MKIVDANVLLYAVNSSAEHHAASQRWLDGALGGGDIVGLAWVPLLAFIRLATKHGLFPSPLRPEEATAQVTEWCHAPGAV
VVNPTPRHSDVLAGLLAKVGTGGNLVNDAHLAALAVEHRASIVSYDSDFGRFDGPRWTTPDALLS

Nucleotide

• Length: 438 bp

>T36329 NZ_AP022606:c106265-105828 [Mycobacterium branderi]
ATGAAGATCGTCGACGCCAATGTTCTTCTCTACGCAGTGAATTCGTCTGCCGAGCATCACGCAGCCAGCCAGCGTTGGCT
CGACGGCGCACTCGGGGGTGGCGATATCGTCGGCCTGGCGTGGGTTCCGCTGCTCGCCTTCATCCGGCTCGCCACCAAGC
ACGGCCTGTTTCCGTCACCGCTTCGTCCAGAGGAGGCAACGGCACAGGTCACGGAGTGGTGCCATGCTCCCGGCGCTGTC
GTGGTCAATCCGACGCCGCGGCACTCCGACGTGCTCGCGGGGTTGCTGGCCAAGGTGGGCACCGGCGGGAACCTGGTCAA
CGACGCGCACCTGGCGGCGCTTGCGGTCGAGCACCGGGCCAGCATCGTCAGCTATGACAGCGACTTCGGGCGCTTCGACG
GCCCACGCTGGACGACGCCCGACGCATTGCTGTCTTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9452.92 Da
• Isoelectric Point: 10.9886

>AT36329 WP_062539743.1 NZ_AP022606:c106516-106262 [Mycobacterium branderi]
MRTTVTLDDDTVALVHRLMKERGVSFKKALNDAIREGAKRRVKPPAFKTRTADLGVPAVNLDRALQVAAELEDEELIRRQ
RRGA

Nucleotide

• Length: 255 bp

>AT36329 NZ_AP022606:c106516-106262 [Mycobacterium branderi]
ATGCGCACGACGGTGACGCTCGACGATGACACGGTGGCGCTCGTGCACCGCTTGATGAAGGAACGTGGCGTGTCCTTCAA
GAAAGCCCTCAACGATGCCATCCGCGAAGGCGCCAAGCGGCGTGTGAAGCCGCCCGCATTCAAGACCCGCACCGCCGACC
TGGGTGTGCCGGCGGTGAACCTTGACCGGGCACTCCAGGTTGCCGCAGAGCTTGAAGACGAGGAATTGATCCGCCGTCAG
CGTCGGGGCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1X1RUF8